Janelia Fluor® 646, free acid
Tocris Bioscience, part of Bio-Techne | Catalog # 8915
Red dye (Reactive group: free acid). Application: flow cytometry, confocal microscopy, super resolution microscopy (SRM) including dSTORM & STED. Suitable for live cell imaging
Key Product Details
Description
Red dye (Reactive group: free acid). Application: flow cytometry, confocal microscopy, super resolution microscopy (SRM) including dSTORM & STED. Suitable for live cell imaging
Product Description
Key Information: Janelia Fluor® 646, free acid is a red fluorogenic fluorescent dye; supplied with a free acid reactive group. Suitable for live cell imaging.Application: Suitable for flow cytometry, confocal microscopy, super resolution microscopy (SRM) including dSTORM (in both live and fixed cells) and STED. Used in protocol (2017 Grimm et al - see references below) for the synthesis of Janelia Fluor® HaloTag® and SNAP-Tag® ligands, for use in live cell imaging experiments. Cell permeable.
Properties and Photophysical Data: Excitation and emission maxima (λ) are 646 nm and 664 nm, respectively; quantum yield = 0.54; extinction coefficient = 152,000 M-1cm-1. A280 correction factor = 0.19; lactone-zwitterion equilibrium constant (KL-Z) = 0.0012.
To measure the absorbance spectrum of this dye we recommend the following solvent: ethanol or trifluoroethanol plus 0.1% TFA.
We also offer custom conjugation antibody services.
HaloTag® is a registered trademark of Promega Corporation, and SNAP-tag® is a registered trademark of New England BioLabs, Inc.
Janelia Fluor® is a registered trademark of Howard Hughes Medical Institute.
This product will replace Janelia Fluor® 646, free acid (Cat. No. 6993).
Licensing Information
Sold under license from the Howard Hughes Medical Institute, Janelia Research CampusUsage Guidelines
This product is provided for use in onward chemistry. Suitable solvents can be used.Optical Data
| Emission Color | Red |
| λabs | 646 nm |
| λem | 664 nm |
| Extinction Coefficient (ε) | 152,000 M-1cm-1 |
| Quantum Yield (φ) | 0.54 |
| Closest Laser line | 640 nm |
| Reactive Group | Free acid |
| Reactivity | Primary amines |
| Cell Permeable | Yes |
| Correction Factor 280 | 0.19 |
| Application | Flow Cytometry, Confocal Microscopy, dSTORM, STED, Live-cell Imaging |
| Alternative For | Alexa Fluor® 647, Atto 647, Atto 647N, CF640R, CF647, Cyanine 5 |
Trademarks and registered trademarks are the property of their respective owners.
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Product Specifications
Molecular Weight
496.64
Formula
C29H28N2O4Si
Storage
Store at -20°C
Purity
≥95% (HPLC)
Chemical Name
1-[7-(1-Azetidinyl)-10-(2,5-dicarboxyphenyl)-9,9-dimethyl-9-silaanthracen-2(9H)-ylidene]azetidinium inner salt
CAS Number
1811539-57-7
PubChem ID
140772579
InChI Key
YIPCOENULPIGPZ-UHFFFAOYSA-N
SMILES
OC(C1=CC=C(C([O-])=O)C(C(C2=CC=C(N3CCC3)C=C2[Si]4(C)C)=C(C=C/5)C4=CC5=[N+]6CCC\6)=C1)=O
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
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Background References
References are publications that support the biological activity of the product.
- Grimm A general method to improve fluorophores for live-cell and single-molecule microscopy. Nat.Methods 2015 PMID: 25599551
- Zheng Rational design of fluorogenic and spontaneously blinking labels for super-resolution imaging. ACS Cent.Sci. 2019 PMID: 31572787
- Grimm Synthesis of Janelia Fluor HaloTag and SNAP-Tag ligands and their use in cellular imaging experiments. Methods Mol.Biol. 2017 PMID: 28924668
Product Specific Notices for Janelia Fluor® 646, free acid
For research use only
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