Janelia Fluor® 635, Tetrazine
Tocris Bioscience, part of Bio-Techne | Catalog # 8134
Red dye (Reactive group: tetrazine). Application: confocal microscopy, super resolution microscopy including dSTORM & STED. Suitable for live cell imaging
Key Product Details
Description
Red dye (Reactive group: tetrazine). Application: confocal microscopy, super resolution microscopy including dSTORM & STED. Suitable for live cell imaging
Product Description
Key Information: Janelia Fluor® 635, Tetrazine is a red fluorogenic fluorescent dye; supplied with a tetrazine reactive handle for copper-free click chemistry. Suitable for live-cell imaging.Application: Suitable for flow cytometry, confocal microscopy, super resolution microscopy (SRM) techniques including dSTORM (in both live and fixed cells). Janelia Fluor® 635, Tetrazine is cell permeable.
Properties and Photophysical Data: Excitation and emission maxima (λ) are 645 nm and 663 nm, respectively.
Janelia Fluor® is a registered trademark of Howard Hughes Medical Institute.
Licensing Information
Sold under license from the Howard Hughes Medical Institute, Janelia Research CampusOptical Data
| Emission Color | Red |
| λabs | 635 nm |
| λem | 652 nm |
| Cell Permeable | Yes |
| Application | Confocal Microscopy, Super Resolution Microscopy, Flow Cytometry |
| Alternative For | Alexa Fluor® 633, Atto 633, CF633 |
Trademarks and registered trademarks are the property of their respective owners.
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectra Viewer
Product Specifications
Molecular Weight
701.81
Formula
C38H33F2N7O3Si
Storage
Store at -20°C
Purity
≥90% (HPLC)
Chemical Name
4-((4-(1,2,4,5-Tetrazin-3-yl)benzyl)carbamoyl)-2-(3-(3-fluoroazetidin-1-ium-1-ylidene)-7-(3-fluoroazetidin-1-yl)-5,5-dimethyl-3,5-dihydrodibenzo[b,e]silin-10-yl)benzoate
InChI Key
DVDISOMTYSQEME-UHFFFAOYSA-N
SMILES
C[Si](C1=C/C2=[N+]3CC(C/3)F)(C4=CC(N5CC(C5)F)=CC=C4C(C6=CC(C(NCC7=CC=C(C8=NN=CN=N8)C=C7)=O)=CC=C6C([O-])=O)=C1C=C2)C
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 7.02 | 10 |
Preparing Stock Solutions
for Janelia Fluor® 635, Tetrazine
The following data is based on the product molecular weight 701.81
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 14.25 mL | 71.24 mL | 142.49 mL |
| 0.5 mM | 2.85 mL | 14.25 mL | 28.50 mL |
| 1 mM | 1.42 mL | 7.12 mL | 14.25 mL |
| 5 mM | 0.28 mL | 1.42 mL | 2.85 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Peng and Hang Site-specific bioorthogonal labeling for fluorescence imaging of intracellular proteins in living cells. J.Am.Chem.Soc. 2016 PMID: 27768298
Product Specific Notices for Janelia Fluor® 635, Tetrazine
For research use only
Loading...
Loading...