Janelia Fluor® 630b, Maleimide
Tocris Bioscience, part of Bio-Techne | Catalog # 8154
Blinking fluorescent dye (Reactive group: maleimide). Application: super resolution microscopy (SRM) including SMLM
Key Product Details
Description
Blinking fluorescent dye (Reactive group: maleimide). Application: super resolution microscopy (SRM) including SMLM
Product Description
Spontaneously Blinking Janelia Fluor® Dyes allow facile single-molecule localization microscopy (SMLM) in cells and dense biomolecular structures, without the need for photoactivation or redox buffers. These spontaneously blinking dyes harness Janelia Fluor® technology to delivery dyes that automatically cycle between 'off' and 'on' states with an ideal duty cycle for super resolution microscopy experiments.Key Information: Janelia Fluor® 630b, Maleimide is a spontaneously blinking fluorescent dye, supplied with a maleimide reactive group for conjugation (thiol reactivity). Suitable to characterize the physical properties of dense biomolecule condensates and complex macromolecular assemblies.
Application: suitable for super resolution microscopy (SRM) including single-molecule localization microscopy (SMLM).
Properties and Photophysical Data: Excitation and emission maxima (λ) are 651 nM and 666 nM, respectively.
Janelia Fluor® is a registered trademark of Howard Hughes Medical Institute.
Licensing Information
Sold under license from the Howard Hughes Medical Institute, Janelia Research CampusOptical Data
| λabs | 651 nm |
| λem | 666 nm |
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Product Specifications
Molecular Weight
760.95
Formula
C37H40N4O8S2Si
Storage
Store at -20°C
Purity
≥90% (HPLC)
Chemical Name
N-(2-(2,5-Dioxo-2,5-dihydro-1H-pyrrol-1-yl)ethyl)-5,5-dimethyl-3,7-bis(3-(methylsulfonyl)azetidin-1-yl)-3'H,5H-spiro[dibenzo[b,e]siline-10,1'-isobenzofuran]-6'-carboxamide
InChI Key
MUANOMDFTIBBQQ-UHFFFAOYSA-N
SMILES
C[Si]1(C)C2=CC(N3CC(S(C)(=O)=O)C3)=CC=C2C4(C(C=C(C(NCCN5C(C=CC5=O)=O)=O)C=C6)=C6CO4)C(C1=C7)=CC=C7N8CC(S(C)(=O)=O)C8
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
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Background References
References are publications that support the biological activity of the product.
- Holland A series of spontaneously blinking dyes for super-resolution microscopy. bioRxiv 2024 PMID: 38766149
Product Specific Notices for Janelia Fluor® 630b, Maleimide
For research use only
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