Janelia Fluor® 549, Maleimide
Tocris Bioscience, part of Bio-Techne | Catalog # 6500
Yellow dye (Reactive group: maleimide). Application: flow cytometry, confocal microscopy, super resolution microscopy including dSTORM & STED. Suitable for live cell imaging
Key Product Details
Description
Yellow dye (Reactive group: maleimide). Application: flow cytometry, confocal microscopy, super resolution microscopy including dSTORM & STED. Suitable for live cell imaging
Product Description
Key information: Janelia Fluor® 549, Maleimide is a yellow fluorescent dye, supplied with a maleimide reactive group for conjugation (thiol reactivity). Suitable for live cell imaging.Application: Suitable for flow cytometry, confocal microscopy, super resolution microscopy (SRM) including dSTORM (in both live and fixed cells) and STED. Cell permeable.
Properties and Photophysical Data: Excitation and emission maxima (λ) are 549 nm and 571 nm, respectively; quantum yield = 0.88; extinction coefficient = 101,000 M-1cm-1; A280 correction factor = 0.169; lactone-zwitterion equilibrium constant (KL-Z) = 3.5.
Please see the protocol for further information and a guide to protein/antibody labeling.
Janelia Fluor® is a registered trademark of Howard Hughes Medical Institute.
Licensing Information
Sold under license from the Howard Hughes Medical Institute, Janelia Research CampusOptical Data
| Emission Color | Yellow |
| Brightness | |
| λabs | 549 nm |
| λem | 571 nm |
| Extinction Coefficient (ε) | 101,000 M-1cm-1 |
| Quantum Yield (φ) | 0.88 |
| Closest Laser line | 532 nm |
| Reactive Group | Maleimide |
| Reactivity | Thiols |
| Cell Permeable | Yes |
| Correction Factor 280 | 0.169 |
| Application | Confocal Imaging, dSTORM, STED, Flow Cytometry |
| Alternative For | Alexa Fluor® 546, Alexa Fluor® 555, BDY TMR-X, Atto 550, CF 555, TAMRA, Cyanine 3 |
Trademarks and registered trademarks are the property of their respective owners.
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Product Specifications
Molecular Weight
690.63
Formula
C33H28N4O6.C2HF3O2
Storage
Store at -20°C
Purity
≥90% (HPLC)
Chemical Name
2-(3-(Azetidin-1-ium-1-ylidene)-6-(azetidin-1-yl)-3H-xanthen-9-yl)-4-((2-(2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)ethyl)carbamoyl)benzoate trifluoroacetate
InChI Key
UOOARLKDIKQOPO-UHFFFAOYSA-N
SMILES
O=C(NCCN1C(C=CC1=O)=O)C2=CC=C(C([O-])=O)C(C(C3=CC=C(N4CCC4)C=C3O5)=C(C=C/6)C5=CC6=[N+]7CCC\7)=C2.FC(C(O)=O)(F)F
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 69.06 | 100 |
Preparing Stock Solutions
for Janelia Fluor® 549, Maleimide
The following data is based on the product molecular weight 690.63
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.45 mL | 7.24 mL | 14.48 mL |
| 5 mM | 0.29 mL | 1.45 mL | 2.90 mL |
| 10 mM | 0.14 mL | 0.72 mL | 1.45 mL |
| 50 mM | 0.03 mL | 0.14 mL | 0.29 mL |
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Background References
References are publications that support the biological activity of the product.
- Zheng Rational design of fluorogenic and spontaneously blinking labels for super-resolution imaging. ACS Cent.Sci. 2019 PMID: 31572787
- Grimm A general method to improve fluorophores for live-cell and single-molecule microscopy. Nat.Methods. 2015 PMID: 25599551
Product Specific Notices for Janelia Fluor® 549, Maleimide
For research use only
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