IRDye® 800CW, NHS ester
Tocris Bioscience, part of Bio-Techne | Catalog # 8881
Near-infared dye (reactive group NHS ester)
Key Product Details
Description
Near-infared dye (reactive group NHS ester)
Product Description
Key information: IRDye® 800CW, NHS ester is a near-infrared (NIR) fluorescent dye, supplied with an NHS ester reactive group for the labeling of primary amines.Application: Fluorescence microscopy.
Properties and Photophysical Data: Excitation and emission maxima (λ) are 773 nm and 792 nm, respectively.
Please see the protocol for further information on protein/antibody labeling and conjugation.
It is recommended to dissolve this dye in water or DMSO (10 - 20 mg/mL)
NOTE: When labeling a peptide or protein with IRDye® 800CW, NHS ester, the choice of solvent for the dye is dependent on the stability of the target. Since a small amount of dye is needed to label large (i.e. >45 kDa) proteins, the amount of DMSO in the reaction is negligible and should not affect protein stability. However, since a proportionally larger amount of dye is required to achieve adequate labeling for a small protein (i.e.
IRDye® is a registered trademark of Li-Cor and is used under license.
Optical Data
| λabs | 773 nm |
| λem | 792 nm |
| Reactive Group | NHS ester |
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Product Specifications
Molecular Weight
1166.20
Formula
C50H54N3Na3O17S4
Storage
Store at -20°C
Purity
≥80% (HPLC)
Chemical Name
Sodium 2-((E)-2-((E)-3-(2-((E)-1-(6-((2,5-Dioxopyrrolidin-1-yl)oxy)-6-oxohexyl)-3,3-dimethyl-5-sulfonatoindolin-2-ylidene)ethylidene)-2-(4-sulfonatophenoxy)cyclohex-1-en-1-yl)vinyl)-3,3-dimethyl-1-(4-sulfonatobutyl)-3H-indol-1-ium-5-sulfonate
CAS Number
956579-01-4
InChI Key
PBPMREYPAOZRBU-UHFFFAOYSA-K
SMILES
CC1(C)C2=CC(S(=O)(O[Na])=O)=CC=C2[N+](CCCCS([O-])(=O)=O)=C1/C=C/C3=C(OC4=CC=C(S(=O)(O[Na])=O)C=C4)/C(CCC3)=C/C=C5C(C)(C)C(C=C(S(=O)(O[Na])=O)C=C6)=C6N\5CCCCCC(ON7C(CCC7=O)=O)=O
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
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Background References
References are publications that support the biological activity of the product.
- Sever Detection properties of indium-111 and IRDye800CW for intraoperative molecular imaging use across tissue phantom models. J.Biomed.Opt. 2025 PMID: 39310036
Product Specific Notices for IRDye® 800CW, NHS ester
For research use only
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