IRDye^® 800CW, DBCO

Key information: IRDye^® 800CW, DBCO is a near-infrared (NIR) fluorescent dye, supplied with a DBCO reactive group for conjugation. DBCO reactive group can react with azide-tagged molecules or biomolecules via copper-free click chemistry.

Application: Fluorescence microscopy.

Properties and Photophysical Data: Excitation and emission maxima (λ) are 778 nm and 794 nm, respectively.

It is recommended to dissolve this dye in water or DMSO (10 - 20 mg/mL). Dye solubilized in aqueous solutions or in DMSO is stable for up to two weeks when stored at -20 °C and protected from light.

DBCO technology enables spontaneous labeling of azide groups in the presence of naturally occuring nucleophiles (e.g. amines and hydroxyls) at physiological pH and temperature. At higher pH or temperature, competition from naturally occuring nucleophiles may occur.

NOTE: When labeling a peptide or protein with IRDye^® 800CW DBCO, the choice of solvent for the dye is dependent on the stability of the target. Since a small amount of dye is needed to label large (i.e. >45 kDa) proteins, the amount of DMSO in the reaction is negligible and should not affect protein stability. However, since a proportionally larger amount of dye is required to achieve adequate labeling for a small protein (i.e. <45 kDa) or peptide, the level of DMSO in the reaction is more likely to affect the target stability. Dye solubilized in aqueous solutions or in DMSO is stable for up to two weeks when stored at -20 °C and protected from light.

IRDye^® is a registered trademark of Li-Cor and is used under license.