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Fluorogenic, green-emitting DNA probe
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Key Product Details
| Description: | Fluorogenic, green-emitting DNA probe |
| Alternative Names: | JF526-HST |
| Chemical Name: | 2-(3-(3,3-Difluoroazetidin-1-ium-1-ylidene)-6-(3,3-difluoroazetidin-1-yl)-2,7-difluoro-3H-xanthen-9-yl)-4-((2-(2-(2-(4-(4-(6-(4-methylpiperazin-1-yl)-1H,3'H-[2,5'-bibenzo[d]imidazol]-2'-yl)phenoxy)butanamido)ethoxy)ethoxy)ethyl)carbamoyl)benzoate tetratrifluoroacetate |
| Purity: | ≥95% (HPLC) |
| Molecular Weight: | 1641.27 |
Biological Activity for Hoechst Janelia Fluor® 526
Hoechst Janelia Fluor® 526 is a fluorogenic, green-emitting DNA probe. Preferentially stains and binds minor groove of AT-rich regions. When bound to A-T DNA, Hoechst Janelia Fluor® 526 exhibits a quantum yield (QY) of 0.126; when bound to G-C DNA Hoechst Janelia Fluor® 526 exhibits a quantum yield = 0.04.
Fluorogenic: fluoresces only once bound to DNA, enabling hassle-free no-wash experiments. Hoechst Janelia Fluor® 526 is suitable for multicolor microscopy experiments and for use in live cell imaging.
Excitation maximum = 531 nm; emission maximum = 549 nm.
To measure the absorbance spectrum of Hoechst Janelia Fluor® 526 we recommend the following solvent: TFE plus 0.1% TFA.
Licensing Information
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus
Optical Data for Hoechst Janelia Fluor® 526
| Emission Color | Yellow |
|---|---|
| λabs | 531 nm |
| λem | 549 nm |
| Extinction Coefficient (ε) | 118,000 M-1cm-1 |
| Quantum Yield (φ) | 0.126 |
| Application | Confocal Microscopy, Super Resolution Microscopy (SRM) |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectra Viewer
Citations for Hoechst Janelia Fluor® 526
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