HBC 530
Tocris Bioscience, part of Bio-Techne | Catalog # 7277
GFP fluorophore mimic for imaging RNA in live cells; activated by binding to Pepper aptamers; suitable for confocal and two-photon microscopy
Key Product Details
Description
GFP fluorophore mimic for imaging RNA in live cells; activated by binding to Pepper aptamers; suitable for confocal and two-photon microscopy
Product Description
HBC 530 is a mimic of green fluorescent protein (GFP) fluorophore for imaging RNA in live cells. Fluoresces when bound to Pepper aptamer; displays high affinity binding (Kd = 3.5 nM). Suitable for confocal and two-photon microscopy. Confocal excitation maximum = 488 nm, emission maximum = 495 - 595 nm. Two-photon excitation maximum = 976 nm, emission maximum = 475 - 575 nm.To make a 1000x stock solution, add 200 μL DMSO (Cat. No. 3176).
Optical Data
| λabs | 485 nm |
| λem | 530 nm |
| Extinction Coefficient (ε) | 65300 M-1cm-1 |
| Quantum Yield (φ) | 0.66 |
| Closest Laser line | 488 nm |
| Application | Fluorogen, used with RNA Light-up Aptamer, Confocal Microscopy |
| Alternative For | Cat. No. 6434 |
Spectra Viewer
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Product Specifications
Molecular Weight
303.37
Formula
C19H17N3O
Storage
Store at -20°C
Purity
≥98% (HPLC)
Chemical Name
4-Cyano-α-[[4-[(2-hydroxyethyl)methylamino]phenyl]methylene]benzeneacetonitrile
CAS Number
156840-13-0
InChI Key
DIAVZHWDYXQAFC-LDADJPATSA-N
SMILES
CN(CCO)C1=CC=C(C=C1)/C=C(C#N)/C2=CC=C(C=C2)C#N
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 30.34 | 100 |
Preparing Stock Solutions
for HBC 530
The following data is based on the product molecular weight 303.37
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 3.30 mL | 16.48 mL | 32.96 mL |
| 5 mM | 0.66 mL | 3.30 mL | 6.59 mL |
| 10 mM | 0.33 mL | 1.65 mL | 3.30 mL |
| 50 mM | 0.07 mL | 0.33 mL | 0.66 mL |
Calculators
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Background References
References are publications that support the biological activity of the product.
- Chen Visualizing RNA dynamics in live cells with bright and stable fluorescent RNAs. Nat.Biotechnol. 2019 PMID: 31548726
Product Specific Notices for HBC 530
For research use only
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