H2DCFDA
Tocris Bioscience, part of Bio-Techne | Catalog # 5935
Fluorescent ROS indicator; cell permeable
Key Product Details
Description
Fluorescent ROS indicator; cell permeable
Product Description
H2DCFDA is a fluorescent ROS indicator; becomes fluorescent when oxidized. Excitation wavelength ~490 nm, emission ~520 nm. Cell permeable.Spectra Viewer
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Spectra ViewerProduct Specifications
Molecular Weight
487.29
Formula
C24H16Cl2O7
Storage
Store at -20°C
Purity
≥95% (HPLC)
Chemical Name
2-[3,6-Bis(acetyloxy)-2,7-dichloro-9H-xanthen-9-yl]benzoic acid
CAS Number
4091-99-0
PubChem ID
77718
InChI Key
PXEZTIWVRVSYOK-UHFFFAOYSA-N
SMILES
ClC1=CC(C(C2=CC=CC=C2C(O)=O)C(C=C(Cl)C(OC(C)=O)=C3)=C3O4)=C4C=C1OC(C)=O
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 48.73 | 100 | |
| Ethanol | 4.87 | 10 |
Preparing Stock Solutions
for H2DCFDA
The following data is based on the product molecular weight 487.29
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.05 mL | 10.26 mL | 20.52 mL |
| 5 mM | 0.41 mL | 2.05 mL | 4.10 mL |
| 10 mM | 0.21 mL | 1.03 mL | 2.05 mL |
| 50 mM | 0.04 mL | 0.21 mL | 0.41 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Santabárbara-Ruiz ROS-induced JNK and p38 signaling is required for unpaired cytokine activation during drosophila regeneration. PLoS Genet. 2015 PMID: 26496642
- Nicholson Sigma-2 receptors play a role in cellular metabolism: stimulation of glycolytic hallmarks by CM764 in human SK-N-SH neuroblastoma. J.Pharmacol.Exp.Ther. 2013 PMID: 26574517
Product Specific Notices for H2DCFDA
For research use only
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