GF-AFC
Tocris Bioscience, part of Bio-Techne | Catalog # 8143
Cell permeable fluorogenic substrate cleaved by cytosolic aminopeptidases for non-lytic high throughput cell viability assays
Key Product Details
Description
Cell permeable fluorogenic substrate cleaved by cytosolic aminopeptidases for non-lytic high throughput cell viability assays
Product Description
GF-AFC (glycyl-phenylalanyl-aminofluorocoumarin) is a cell permeable peptide fluorogenic substrate. GF-AFC is cleaved by cytosolic aminopeptidases for non-lytic high throughput cell viability assays. GF-AFC can be used as a substrate for measuring the activity of dipeptidyl peptidase 1 (DPP1), an intracellular lysosomal cysteinyl protease.Spectra Viewer
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Product Specifications
Molecular Weight
469.85
Formula
C21H18F3N3O4.HCl
Storage
Store at -20°C
Purity
≥98% (HPLC)
Chemical Name
(2S)-2-[(2-Aminoacetyl)amino]-N-[2-oxo-4-(trifluoromethyl)chromen-7-yl]-3-phenylpropanamide hydrochloride
InChI Key
GBCCOIYISILSJP-NTISSMGPSA-N
SMILES
NCC(N[C@@H](CC1=CC=CC=C1)C(NC2=CC=C(C(O3)=C2)C(C(F)(F)F)=CC3=O)=O)=O.Cl
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 46.98 | 100 |
Preparing Stock Solutions
for GF-AFC
The following data is based on the product molecular weight 469.85
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.13 mL | 10.64 mL | 21.28 mL |
| 5 mM | 0.43 mL | 2.13 mL | 4.26 mL |
| 10 mM | 0.21 mL | 1.06 mL | 2.13 mL |
| 50 mM | 0.04 mL | 0.21 mL | 0.43 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Niles A homogeneous assay to measure live and dead cells in the same sample by detecting different protease markers. Anal.Biochem. 2007 PMID: 17512890
- Liu Serum based fluorescent assay for evaluating dipeptidyl peptidase I activity in collagen induced arthritis rat model. Mol.Cell.Probes 2017 PMID: 27771442
Product Specific Notices for GF-AFC
For research use only
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