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FURA-2AM

Tocris Bioscience a Bio-Techne Brand

FURA-2AM | CAS No. 108964-32-5 | Fluorescent Ca2+ indicator
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2220/1
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Key Product Details

Description: Fluorescent Ca2+ indicator
Chemical Name: 2-[6-[bis[2-[(Acetyloxy)methoxy]-2-oxoethyl]amino]-5-[2-[2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]-2-benzofuranyl]-5-oxazolecarboxylic acid (acetyloxy)methyl ester
Purity: ≥95% (HPLC)
Molecular Weight: 1001.85

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Citations for FURA-2AM (3)

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Customer Reviews for FURA-2AM (3)

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  • Detects store-operated Ca2+ entry after ER store depletion in RBL-2H3 cells.
    Name: Niroj Shrestha
    Species: Rat
    Assay Type: In Vitro
    Cell Line/Tissue: RBL-2H3
    RBL-2H3 cells at a confluency of 50–60% were cultured on glass coverslip and loaded with 2 μM Fura-2AM in normal tyrode solution (NT) for 40 min at room temperature in the dark. Then, the cells were washed twice and left to equilibrate for at least 20 min in NT. The Fura-2 loaded cells were excited alternately at 340 and 380 nm and fluorescence was captured at 510 nm every 1.2 s. ER Ca2+ store was depleted with 500 nM thapsigargin which promotes store-operated Ca2+ entry into the cells upon addition of 2 mM extracellular Ca2+.
    FURA-2AM Serum 2220
  • FURA-2AM as a tool to analyse calcium transient in cardiomyocytes
    Name: Christopher Trummer
    Species: Other
    Assay Type: In Vitro
    Cell Line/Tissue: primary ventricular cardiomyocytes
    Primary guinea pig ventricular cardiomyocytes were loaded with FURA-2AM (1 μM) and Pluronic F 127 (1%) for 30 min followed by washing with normal-Tyrode solution. Cells were stimulated at 1 Hz frequency and fluorescence intensities were measured.
    FURA-2AM Serum 2220
  • Detects cytosolic calcium influx changes well
    Name: Anonymous
    Species: Rat
    Assay Type: In Vitro
    Cell Line/Tissue: spinal cord neurons
    Used in live cell calcium imaging assay to study glutamate receptor signaling in neurons. Used at a concentration of 5 micromolar (along with pluronic). Images shows changes in fura-2 fluorescence pre (left) and post(right) glutamate stimulation.
    FURA-2AM Serum 2220

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Product Documents for FURA-2AM

Certificate of Analysis

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