Skip to main content

Fluo-4 AM

Tocris Bioscience a Bio-Techne Brand

Fluo-4 AM | CAS No. 273221-67-3 | Cell-permeable, fluorescent Ca2+ indicator
Catalog #
Size / Price
15% Off Limited-time Savings on Your Reagents, Assays and Kits
Now through September 27th, you can save 15% on Bio-Techne reagents, assays, and kits (including Novus, Tocris and R&D Systems). Use promotion code SAVE15 at checkout on orders over $1,000. See terms and conditions for offer details.
Introduction to Tocris

Key Product Details

Description: Cell-permeable, fluorescent Ca2+ indicator
Chemical Name: N-[4-[6-[(Acetyloxy)methoxy]-2,7-difluoro-3-oxo-3H-xanthen-9-yl]-2-[2-[2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]phenyl]-N-[2-[(acetyloxy)methoxy]-2-oxoethyl]glycine (acetyloxy)methyl ester
Purity: ≥95% (HPLC)
Molecular Weight: 1096.95

Citations for Fluo-4 AM

Please visit our Instrument Citation Database to search our collection of scientific articles that feature our instruments.

Customer Reviews for Fluo-4 AM (2)

4.5 out of 5
2 customer ratings
5 stars
4 stars
3 stars
2 stars
1 star

Customer Images

Showing  1 - 2 of 2 reviews Showing All
Filter By:
  • Fluo-4 detects store-operated Ca2+ entry upon ER Ca2+ store depletion in RBL-2H3 cells.
    Name: Niroj Shrestha
    Species: Rat
    Assay Type: In Vitro
    Cell Line/Tissue: RBL-2H3
    RBL-2H3 cells cultured on glass coverslip were loaded with 2 μM Fluo-4AM in normal tyrode solution (NT) for 40 min at room temperature in the dark. Then, the cells were washed twice and left to equilibrate for at least 20 min in NT. Fluo-4-loaded cells were excited at 490 nm and fluorescence was captured at 510 nm every 2.4 s and normalized to the initial value. ER Ca2+ store was depleted with 500 nM thapsigargin which promotes store-operated Ca2+ entry into the cells upon addition of 2 mM extracellular Ca2+.
    Fluo-4 AM Serum 6255
  • works well in motor neurons
    Name: Karthik krishnamurthy
    Species: Rat
    Assay Type: In Vitro
    Cell Line/Tissue: motor neurons
    Used for imaging calcium entry in motor neurons at 1 mM concentration
    motor neurons loaded with 1 mM concentration of fluo4-am loaded at 37 degrees for 15 minutes in aCSF. After 15 minutes cells were gently washed with aCSF twice and left in dark for 10 minutes for complete de-esterification of the dye.
    Fluo-4 AM Serum 6255

There are no reviews that match your criteria.

Showing  1 - 2 of 2 reviews Showing All

Product Documents for Fluo-4 AM

Certificate of Analysis

To download a Certificate of Analysis, please select a batch below.