Fluo-4 AM

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• (2)

• Fluo-4 detects store-operated Ca2+ entry upon ER Ca2+ store depletion in RBL-2H3 cells.

  Name: Niroj Shrestha
  Species: Rat
  Assay Type: In Vitro
  Cell Line/Tissue: RBL-2H3
  RBL-2H3 cells cultured on glass coverslip were loaded with 2 μM Fluo-4AM in normal tyrode solution (NT) for 40 min at room temperature in the dark. Then, the cells were washed twice and left to equilibrate for at least 20 min in NT. Fluo-4-loaded cells were excited at 490 nm and fluorescence was captured at 510 nm every 2.4 s and normalized to the initial value. ER Ca2+ store was depleted with 500 nM thapsigargin which promotes store-operated Ca2+ entry into the cells upon addition of 2 mM extracellular Ca2+.
  

• works well in motor neurons

  Name: Karthik krishnamurthy
  Species: Rat
  Assay Type: In Vitro
  Cell Line/Tissue: motor neurons
  Used for imaging calcium entry in motor neurons at 1 mM concentration
  motor neurons loaded with 1 mM concentration of fluo4-am loaded at 37 degrees for 15 minutes in aCSF. After 15 minutes cells were gently washed with aCSF twice and left in dark for 10 minutes for complete de-esterification of the dye.
  

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