FFN 200 dihydrochloride
Tocris Bioscience, part of Bio-Techne | Catalog # 5911
Selective fluorescent VMAT2 substrate
Key Product Details
Description
Selective fluorescent VMAT2 substrate
Product Description
FFN 200 dihydrochloride is a selective fluorescent VMAT2 substrate. Selectively traces exocytosis in dopaminergic neuronal cell culture and brain tissue. Excitation and emission maxima are 352 and 451 nm, respectively.Scientific Data Images for FFN 200 dihydrochloride
Application of FFN 200 dihydrochloride in Cultured Dopamine Neurons.
FFN 200 dihydrochloride in vesicle clusters in axons of cultured dopaminergic neurons (top image), and exocytosis upon high potassium stimulation (bottom image).Licensing Information
Sold with the permission of Columbia University.Spectra Viewer
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Product Specifications
Molecular Weight
277.15
Formula
C11H12N2O2.2HCl
Storage
Store at -20°C
Purity
≥98% (HPLC)
Chemical Name
4-(2-Aminoethyl)-7-amino-2H-1-benzopyran-2-one dihydrochloride
CAS Number
2080306-27-8
PubChem ID
124201850
InChI Key
YNQXRSNAOWBGLD-UHFFFAOYSA-N
SMILES
NC1=CC(OC(C=C2CCN)=O)=C2C=C1.Cl.Cl
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| water | 27.71 | 100 | |
| DMSO | 27.71 | 100 |
Preparing Stock Solutions
for FFN 200 dihydrochloride
The following data is based on the product molecular weight 277.15
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 3.61 mL | 18.04 mL | 36.08 mL |
| 5 mM | 0.72 mL | 3.61 mL | 7.22 mL |
| 10 mM | 0.36 mL | 1.80 mL | 3.61 mL |
| 50 mM | 0.07 mL | 0.36 mL | 0.72 mL |
Calculators
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Background References
References are publications that support the biological activity of the product.
- Li Probes for monitoring regulated exocytosis. Cell Calcium 2017 PMID: 28089267
- Pereira Fluorescent false neurotransmitter reveals functionally silent DA vesicle clusters in the striatum. Nat.Neurosci. 2015 PMID: 26900925
Product Specific Notices for FFN 200 dihydrochloride
For research use only
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