FAM-DEALAHypYIPMDDDFQLRSF

Name: FAM-DEALAHypYIPMDDDFQLRSF
Brand: Tocris Bioscience
SKU: 7452
Price: 272 USD

Catalog # 7452 | Tocris Bioscience a Bio-Techne Brand

COA / SDS

Fluorescent HIF-1α peptide; can be used to evaluate the effect of VHL binding on degradation activity

Key Product Details

Description:	Fluorescent HIF-1α peptide; can be used to evaluate the effect of VHL binding on degradation activity
Purity:	≥95% (HPLC)
Molecular Weight:	2616.76

View Full Technical Details for FAM-DEALAHypYIPMDDDFQLRSF

Biological Activity for FAM-DEALAHypYIPMDDDFQLRSF

FAM-DEALAHypYIPMDDDFQLRSF is a 5-carboxyfluorescein (FAM) labeled HIF-1α peptide. It binds with high affinity to von Hippel-Lindau (VHL) protein (K_D = 3 nM). Can be used to assess ligand binding to VHL in a direct fluorescence polarization (FP) displacement assay. Excitation/emission maxima (λ) = 485/520 nm. This product is a longer peptide version of FAM-DEALA-Hyp-YIPD (Cat. No. 7287).

Optical Data for FAM-DEALAHypYIPMDDDFQLRSF

Emission Color	Green
λ_abs	485 nm
λ_em	520 nm
Closest Laser line	488 nm
Application	Fluorescence Polarization (FP) Displacement Assays

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Technical Data for FAM-DEALAHypYIPMDDDFQLRSF

M. Wt	2616.76
Formula	C₁₂₂H₁₅₈N₂₄O₃₉S
Single Letter Sequence	DEALAXYIPMDDDFQLRSF (Modifications: Asp-1 = 5-FAM-Asp, X-6 = Hyp, Phe-19 = C-terminal amide)
Storage	Store at -20°C
Purity	≥95% (HPLC)
InChi Key	BWRLQGDMCXAUCD-GMYGBYBYSA-N
Smiles	O=C(C1=CC(C(O2)=O)=C(C=C1)C32C4=CC=C(O)C=C4OC5=C3C=CC(O)=C5)N[C@@H](CC(O)=O)C(N[C@@H](CCC(O)=O)C(N[C@@H](C)C(N[C@@H](CC(C)C)C(N[C@@H](C)C(N6[C@@H](CC[C@H]6O)C(N[C@@H](CC7=CC=C(C=C7)O)C(N[C@@H]([C@@H](C)CC)C(N8[C@@H](CCC8)C(N[C@@H](CCSC)C(N[C@@H](CC(O)=O)C(N[C@@H](CC(O)=O)C(N[C@@H](CC(O)=O)C(N[C@@H](CC9=CC=CC=C9)C(N[C@@H](CCC(N)=O)C(N[C@@H](CC(C)C)C(N[C@@H](CCCNC(N)=N)C(N[C@@H](CO)C(N[C@@H](CC%10=CC=CC=C%10)C(N)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O.O=C(C(C=C%11)=CC(C%12%13C%14=CC=C(O)C=C%14OC%15=C%12C=CC(O)=C%15)=C%11C(O%13)=O)N[C@@H](CC(O)=O)C(N[C@@H](CCC(O)=O)C(N[C@@H](C)C(N[C@@H](CC(C)C)C(N[C@@H](C)C(N%16[C@@H](CC[C@H]%16O)C(N[C@@H](CC%17=CC=C(C=C%17)O)C(N[C@@H]([C@@H](C)CC)C(N%18[C@@H](CCC%18)C(N[C@@H](CCSC)C(N[C@@H](CC(O)=O)C(N[C@@H](CC(O)=O)C(N[C@@H](CC(O)=O)C(N[C@@H](CC%19=CC=CC=C%19)C(N[C@@H](CCC(N)=O)C(N[C@@H](CC(C)C)C(N[C@@H](CCCNC(N)=N)C(N[C@@H](CO)C(N[C@@H](CC%20=CC=CC=C%20)C(N)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for FAM-DEALAHypYIPMDDDFQLRSF

Solubility

Soluble to 1mg/ml in PBS (pH 7.4)

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References for FAM-DEALAHypYIPMDDDFQLRSF

References are publications that support the biological activity of the product.

Crews Identification and Characterization of Von Hippel-Lindau-Recruiting Proteolysis Targeting Chimeras (PROTACs) of TANK-Binding Kinase 1. J.Med.Chem. 2018 PMID: 28692295
Buckley Targeting the von Hippel-Lindau E3 ubiquitin ligase using small molecules to disrupt the VHL/HIF-1a interaction. J.Am.Chem.Soc. 2012 PMID: 22369643
Ahn HIF-1α peptide derivatives with modifications at the hydroxyproline residue as activators of HIF-1α. Bioorg.Med.Chem.Lett. 2009 PMID: 19515556

Citations for FAM-DEALAHypYIPMDDDFQLRSF

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