FAM-DEALA-Hyp-YIPD
Tocris Bioscience, part of Bio-Techne | Catalog # 7287
Fluorescent HIF-1α peptide; can be used to evaluate the effect of VHL binding on degradation activity
Discontinued Product
7287 has been discontinued.
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Key Product Details
Description
Fluorescent HIF-1α peptide; can be used to evaluate the effect of VHL binding on degradation activity
Product Description
FAM-DEALA-Hyp-YIPD is a fluorescent HIF-1α peptide (Kd = 180-560 nM). Can be used to assess VHL binding in Fluorescence Polarization (FP) displacement assay, and evaluate the effect of VHL binding on degradation activity. Excitation maximum = 485 nm, emission maximum = 535 nm. Longer peptide version, FAM-DEALAHypYIPMDDDFQLRSF (Cat. No. 7452), also available.Optical Data
| Emission Color | Green |
| λabs | 485 nm |
| λem | 520 nm |
| Closest Laser line | 488 nm |
| Application | Fluorescence Polarization (FP) Displacement Assays |
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Product Specifications
Molecular Weight
1477.48
Formula
C71H84N10O25
Storage
Store at -20°C
Purity
≥95% (HPLC)
InChI Key
CWNCTVVEODBMBK-RHNKRQRVSA-N
SMILES
O=C([C@@H]1CCCN1C([C@H]([C@@H](CC)C)NC([C@H](CC2=CC=C(O)C=C2)NC([C@@H]3C[C@@H](O)CN3C([C@@H](NC([C@H](CC(C)C)NC([C@@H](NC([C@H](CCC(O)=O)NC([C@@H](NC(C4=CC(C(O5)=O)=C(C=C4)C65C7=CC=C(O)C=C7OC8=C6C=CC(O)=C8)=O)CC(O)=O)=O)=O)C)=O)=O)C)=O)=O)=O)=O)N[C@@H](CC(O)=O)C(O)=O.O=C([C@@H]9CCCN9C([C@H]([C@@H](CC)C)NC([C@H](CC%10=CC=C(O)C=C%10)NC([C@@H]%11C[C@@H](O)CN%11C([C@@H](NC([C@H](CC(C)C)NC([C@@H](NC([C@H](CCC(O)=O)NC([C@@H](NC(C(C=C%12)=CC(C%13%14C%15=CC=C(O)C=C%15OC%16=C%13C=CC(O)=C%16)=C%12C(O%14)=O)=O)CC(O)=O)=O)=O)C)=O)=O)C)=O)=O)=O)=O)N[C@@H](CC(O)=O)C(O)=O
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solubility | Soluble to 1mg/ml in 0.01M PBS |
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Background References
References are publications that support the biological activity of the product.
- Ahn HIF-1α peptide derivatives with modifications at the hydroxyproline residue as activators of HIF-1α. Bioorg.Med.Chem.Lett. 2009 PMID: 19515556
- Crews Identification and characterization of von Hippel-Lindau-recruiting Proteolysis Targeting Chimeras (PROTACs) of TANK-binding kinase 1. J.Med.Chem. 2018 PMID: 28692295
- Lucas Surface probing by fragment-based screening and computational methods identifies ligandable pockets on the von Hippel-Lindau (VHL) E3 ubiquitin ligase. J.Med.Chem. 2018 PMID: 30040896
- Roy SPR-measured kinetics of PROTAC ternary complexes influence target degradation rate. ACS Chem.Biol. 2019 PMID: 30721025
- Buckley Targeting the von Hippel-Lindau E3 ubiquitin ligase using small molecules to disrupt the VHL/HIF-1a interaction. J.Am.Chem.Soc. 2012 PMID: 22369643
Product Specific Notices for FAM-DEALA-Hyp-YIPD
For research use only
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