dTAG Janelia Fluor® 635
Tocris Bioscience, part of Bio-Techne | Catalog # 8101
Fluorogenic srTAG probe for live cell imaging of FBKP12 (F36V/L) fusion proteins
Key Product Details
Description
Fluorogenic srTAG probe for live cell imaging of FBKP12 (F36V/L) fusion proteins
Product Description
Key Information: dTAG Janelia Fluor® 635 is a fluorogenic srTAG probe for live cell imaging of FKBP12F36V/L fusion proteins.Application: Suitable for confocal microscopy and Super Resolution Microscopy (SRM) including STED. dTAG Janelia Fluor® 635 is cell permeable.
Properties and Photophysical Data: In FRAP (fluorescence recovery after photobleaching) experiment, the highest maximum recovery ratio (85%) was shown when U2OS cells expressing FKBPF36L were incubated with 10 μM dTAG Janelia Fluor® 635; excitation and emission maxima (λ) are 640 nm and 665 nm, respectively; quantum yield = 0.41; extinction coefficient = 75,000 M-1cm-1 (in 4% TFA).
Janelia Fluor® is a registered trademark of Howard Hughes Medical Institute.
Scientific Data Images for dTAG Janelia Fluor® 635
Application of dTAG Janelia Fluor® 635.
HEK293 cells were incubated for 1 hour with 2.5 μM dTAG Janelia Fluor® 635 (red) and Hoechst (blue), without (left) or with (right) endogenous protein of interest labeled with dTAG/FKBP12F36V. Fluorescent microscopy images kindly provided by Yongli Shan, Vincinitas Therapeutics.
Application of dTAG Janelia Fluor® 635.
HEK293 cells were incubated for 1 hour with different concentrations of dTAG Janelia Fluor® 635 (red) and Hoechst (blue), without (top) or with (bottom) endogenous protein of interest labeled with dTAG/FKBP12F36V. Images captured by high content imaging were kindly provided by Yongli Shan, Vincinitas Therapeutics.Licensing Information
Sold under license from the Howard Hughes Medical Institute, Janelia Research CampusOptical Data
| Emission Color | Red |
| Brightness | |
| λabs | 640 nm |
| λem | 665 nm |
| Extinction Coefficient (ε) | 75000 M-1cm-1 |
| Quantum Yield (φ) | 0.41 |
| Closest Laser line | 635 nm |
| Cell Permeable | Yes |
| Application | Confocal Microscopy, Super Resolution Microscopy |
Spectra Viewer
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Product Specifications
Molecular Weight
1250.18
Formula
C69H77F2N5O13Si
Storage
Store at -20°C
Purity
≥95% (HPLC)
Chemical Name
4-((2-(2-(3-((R)-3-(3,4-Dimethoxyphenyl)-1-(((S)-1-((S)-2-(3,4,5-trimethoxyphenyl)butanoyl)piperidine-2-carbonyl)oxy)propyl)phenoxy)acetamido)ethyl)carbamoyl)-2-(3-(3-fluoroazetidin-1-ium-1-ylidene)-7-(3-fluoroazetidin-1-yl)-5,5-dimethyl-3,5-dihydrodibenzo[b,e]silin-10-yl)benzoate
InChI Key
UPUINLLKPHUNRA-QXDPIUJBSA-N
SMILES
O=C([C@@H]1CCCCN1C([C@H](C2=CC(OC)=C(OC)C(OC)=C2)CC)=O)O[C@@H](C3=CC(OCC(NCCNC(C4=CC=C(C([O-])=O)C(C(C5=CC=C(N6CC(F)C6)C=C5[Si]7(C)C)=C(C=C/8)C7=CC8=[N+]9CC(F)C\9)=C4)=O)=O)=CC=C3)CCC%10=CC(OC)=C(OC)C=C%10
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 12.5 | 10 |
Preparing Stock Solutions
for dTAG Janelia Fluor® 635
The following data is based on the product molecular weight 1250.18
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 8.00 mL | 39.99 mL | 79.99 mL |
| 0.5 mM | 1.60 mL | 8.00 mL | 16.00 mL |
| 1 mM | 0.80 mL | 4.00 mL | 8.00 mL |
| 5 mM | 0.16 mL | 0.80 mL | 1.60 mL |
Calculators
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Background References
References are publications that support the biological activity of the product.
- Du Self-renewable tag for photostable fluorescence imaging of proteins. J.Am.Chem.Soc. 2024 PMID: 37596976
Product Specific Notices for dTAG Janelia Fluor® 635
For research use only
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