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DRAQ5 (TM) - 0.5 mM

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-81129

Novus Biologicals, part of Bio-Techne
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NBP2-81129

Key Product Details

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Microscopy

Format

Low Concentration

Product Summary for DRAQ5 (TM) - 0.5 mM

Key Features of DRAQ5 (TM) :
Bright, far-red DNA dye that works on live or fixed cell and tissue preparations for multiple applications such as flow cytometry, fluorescence imaging, and high content screening
Proven no-lyse, no-wash method for measuring DNA content in cells
Tested on hundreds of cell and tissue types
Exceptionally photostable so no photobleaching effect
Convenient, ready-to-use formulation
Enables phenotyping of blood/BM and direct cellular DNA content analysis in flow cytometry without any RBC lysis or fixation, permeabilization or RNase treatment
Spectrally compatible with most visible-range fluorophores including GFP/FITC and R-PE

Products are shipped at ambient temperature, but on receipt packs should be stored at 2-8C. DO NOT FREEZE. DRAQ5 (TM) can come out of solution when frozen and it is difficult (but not impossible) to get it back into solution.

Product Specifications

Applications

Flow Cytometry (1:25 - 1:100)
Immunocytochemistry/ Immunofluorescence (1:100)
Live Imaging Microscopy (1:100)
Fluorescence Imaging (1:100)

Application Notes

DRAQ5 (TM) is supplied at a concentration of 0.5 mM for 250 Imaging assays or 100 Flow Cytometry assays. It can be diluted in culture media (e.g. RPMI 1640) and physiological buffers (eg PBS, Hankss, etc.) and mixed with fixatives such as formaldehyde.

Scientific Data Images for DRAQ5 (TM) - 0.5 mM

Immunocytochemistry/ Immunofluorescence: DRAQ5 (TM) - 0.5 mM [NBP2-81129]

Immunocytochemistry/ Immunofluorescence: DRAQ5 (TM) - 0.5 mM [NBP2-81129]

Immunocytochemistry/Immunofluorescence: DRAQ5 (TM) - 0.5 mM [NBP2-81129] - DRAQ5 (TM) (red) counterstaining of fixed U2OS cells. AlexaFluor 488 antibody to beta-tubulin (green).
Flow Cytometry: DRAQ5 (TM) - 0.5 mM [NBP2-81129]

Flow Cytometry: DRAQ5 (TM) - 0.5 mM [NBP2-81129]

Flow Cytometry: DRAQ5 (TM) - 0.5 mM [NBP2-81129] - No lyse, no wash gating of nucleated cells from whole bone marrow gating on DRAQ5 (TM) signal.
Immunocytochemistry/ Immunofluorescence: DRAQ5 (TM) - 0.5 mM [NBP2-81129]

Immunocytochemistry/ Immunofluorescence: DRAQ5 (TM) - 0.5 mM [NBP2-81129]

Immunocytochemistry/Immunofluorescence: DRAQ5 (TM) - 0.5 mM [NBP2-81129] - DRAQ5 (TM) used to stain dsDNA of dividing cells.

Formulation, Preparation, and Storage

Purification

>97%

Concentration

Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at 4C in the dark. Do not freeze.

Background: DRAQ5 (TM) - 0.5 mM

Deep Red Anthraquinone 5 (DRAQ5 (TM)) is a cell-permeable dye belonging to the anthraquinone family that intercalates with high affinity to double stranded DNA (dsDNA). This far-red emitting fluorescent DNA dye is used for cell cycle analysis and nuclear staining. No measurable binding to mitochondrial DNA (mtDNA) has been detected and DRAQ5 (TM) shows negligible binding to RNA.

The excitation wavelength maxima of DRAQ5 (TM) are 600 nm / 646 nm but it can also be suboptimally excited by the 488nm (blue laser) for flow cytometry. Its emission wavelength maximum is 697 nm when intercalated with dsDNA. The fluorescence signal is proportional and stoichiometric to the quantity of nuclear DNA present in the cell.

Alternate Names

DRAQ5

Additional DRAQ5 (TM) - 0.5 mM Products

Product Documents for DRAQ5 (TM) - 0.5 mM

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for DRAQ5 (TM) - 0.5 mM

DRAQ5 (TM) is a registered trademark of BioStatus Limited.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

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