DMHBO+
Tocris Bioscience, part of Bio-Techne | Catalog # 7764
Fluorescent upon binding to Chili aptamer; for imaging RNA in cells
Key Product Details
Description
Fluorescent upon binding to Chili aptamer; for imaging RNA in cells
Product Description
DMHBO+ is a cationic fluorophore. Its fluorescence is activated by binding to chili aptamer (Kd = 12 nM). Chili-DMHBO+ complex mimics red fluorescent proteins, suitable for imaging RNA in cells. It is an ideal FRET donor to the rhodamine dye Atto 590 and has applications in FRET-based analytical RNA systems. Excitation/emission maxima (λ) = 456/592 nm; quantum yield = 0.1; stokes shift = 136 nm.Optical Data
| λabs | 456 nm |
| λem | 592 nm |
| Quantum Yield (φ) | 0.1 |
| Application | Time-resolved fluorescence energy transfer (TR-FRET) |
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectra Viewer
Product Specifications
Molecular Weight
552.37
Formula
C22H25IN4O5
Storage
Store at -20°C
Purity
≥98% (HPLC)
Chemical Name
4-(4-((Z)-4-Hydroxy-3,5-dimethoxybenzylidene)-2-((hydroxyimino)methyl)-5-oxo-4,5-dihydro-1H-imidazol-1-yl)-N,N,N-trimethylbenzenaminium iodide
CAS Number
2322286-81-5
InChI Key
KRIJKRIPLDZXHI-UHFFFAOYSA-N
SMILES
O/N=C/C(N(C1=CC=C(C=C1)[N+](C)(C)C)C/2=O)=NC2=C/C3=CC(OC)=C(C(OC)=C3)O.[I-]
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 27.62 | 50 |
Preparing Stock Solutions
for DMHBO+
The following data is based on the product molecular weight 552.37
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.5 mM | 3.62 mL | 18.10 mL | 36.21 mL |
| 2.5 mM | 0.72 mL | 3.62 mL | 7.24 mL |
| 5 mM | 0.36 mL | 1.81 mL | 3.62 mL |
| 25 mM | 0.07 mL | 0.36 mL | 0.72 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Steinmetzger A multicolor large stokes shift fluorogen-activating RNA aptamer with cationic chromophores. Chemistry 2019 PMID: 30485561
Product Specific Notices for DMHBO+
For research use only
Loading...
Loading...