DFHBI
Tocris Bioscience, part of Bio-Techne | Catalog # 5609
GFP fluorophore mimic for imaging RNA in living cells; activated by binding Spinach2 and Broccoli aptamers
Key Product Details
Description
GFP fluorophore mimic for imaging RNA in living cells; activated by binding Spinach2 and Broccoli aptamers
Product Description
DFHBI is a mimic of green fluorescent protein (GFP) fluorophore for imaging RNA in living cells. DFHBI fluorescence is activated by binding to Spinach2 or Broccoli aptamers. Exhibits peak excitation maxima of 447 nm and peak fluorescence emission of 501 nm when bound to Spinach2.Spectra Viewer
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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Product Specifications
Molecular Weight
252.22
Formula
C12H10F2N2O2
Storage
Store at -20°C
Purity
≥98% (HPLC)
Chemical Name
(5Z)-5-[(3,5-Difluoro-4-hydroxyphenyl)methylene]-3,5-dihydro-2,3-dimethyl-4H-imidazol-4-one
CAS Number
1241390-29-3
PubChem ID
70808995
InChI Key
ZDDIJYXDUBFLID-YHYXMXQVSA-N
SMILES
CN(C2=O)C(C)=N\C2=C/C1=CC(F)=C(O)C(F)=C1
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 25.22 | 100 |
Preparing Stock Solutions
for DFHBI
The following data is based on the product molecular weight 252.22
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 3.96 mL | 19.82 mL | 39.65 mL |
| 5 mM | 0.79 mL | 3.96 mL | 7.93 mL |
| 10 mM | 0.40 mL | 1.98 mL | 3.96 mL |
| 50 mM | 0.08 mL | 0.40 mL | 0.79 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Truong The fluorescent aptamer Squash extensively repurposes the adenine riboswitch fold. Nat.Chem.Biol. 2022 PMID: 34937911
- Svensen and Jaffrey Fluorescent RNA Aptamers as a Tool to Study RNA-Modifying Enzymes. Cell Chem.Biol. 2016 PMID: 26877022
- Paige RNA mimics of green fluorescent protein. Science 2011 PMID: 21798953
- Strack Using Spinach-based sensors for fluorescence imaging of intracellular metabolites and proteins in living bacteria. Nat.Protoc. 2014 PMID: 24356773
- Filonov In-gel imaging of RNA processing using broccoli reveals optimal aptamer expression strategies. Chem.Biol. 2015 PMID: 26000751
- Song Plug-and-play fluorophores extend the spectral properties of Spinach. J.Am.Chem.Soc. 2014 PMID: 24393009
Product Specific Notices for DFHBI
For research use only
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