DFHBI

Name: DFHBI
Brand: Tocris Bioscience
SKU: 5609

Tocris Bioscience, part of Bio-Techne | Catalog # 5609

Citations (7)

COA / SDS

GFP fluorophore mimic for imaging RNA in living cells; activated by binding Spinach2 and Broccoli aptamers

Key Product Details

Description:	GFP fluorophore mimic for imaging RNA in living cells; activated by binding Spinach2 and Broccoli aptamers
Chemical Name:	(5Z)-5-[(3,5-Difluoro-4-hydroxyphenyl)methylene]-3,5-dihydro-2,3-dimethyl-4H-imidazol-4-one
Purity:	≥98% (HPLC)
Molecular Weight:	252.22

View Full Technical Details for DFHBI

Technical Data
Citations

Biological Activity for DFHBI

DFHBI is a mimic of green fluorescent protein (GFP) fluorophore for imaging RNA in living cells. DFHBI fluorescence is activated by binding to Spinach2 or Broccoli aptamers. Exhibits peak excitation maxima of 447 nm and peak fluorescence emission of 501 nm when bound to Spinach2.

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Technical Data for DFHBI

M. Wt	252.22
Formula	C₁₂H₁₀F₂N₂O₂
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	1241390-29-3
PubChem ID	70808995
InChi Key	ZDDIJYXDUBFLID-YHYXMXQVSA-N
Smiles	CN(C2=O)C(C)=N\C2=C/C1=CC(F)=C(O)C(F)=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for DFHBI

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	25.22	100

Preparing Stock Solutions for DFHBI

The following data is based on the product molecular weight 252.22.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.96 mL	19.82 mL	39.65 mL
5 mM	0.79 mL	3.96 mL	7.93 mL
10 mM	0.40 mL	1.98 mL	3.96 mL
50 mM	0.08 mL	0.40 mL	0.79 mL

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References for DFHBI

References are publications that support the biological activity of the product.

Truong The fluorescent aptamer Squash extensively repurposes the adenine riboswitch fold. Nat.Chem.Biol. 2022 PMID: 34937911
Svensen and Jaffrey Fluorescent RNA Aptamers as a Tool to Study RNA-Modifying Enzymes. Cell Chem.Biol. 2016 PMID: 26877022
Paige RNA mimics of green fluorescent protein. Science 2011 PMID: 21798953
Strack Using Spinach-based sensors for fluorescence imaging of intracellular metabolites and proteins in living bacteria. Nat.Protoc. 2014 PMID: 24356773
Filonov In-gel imaging of RNA processing using broccoli reveals optimal aptamer expression strategies. Chem.Biol. 2015 PMID: 26000751
Song Plug-and-play fluorophores extend the spectral properties of Spinach. J.Am.Chem.Soc. 2014 PMID: 24393009