DAF FM diacetate
Tocris Bioscience, part of Bio-Techne | Catalog # 7756
Cell permeable photostable nitric oxide (NO) fluorescent indicator
Key Product Details
Description
Cell permeable photostable nitric oxide (NO) fluorescent indicator
Product Description
DAF-FM DA is a cell-permeable fluorescent probe for the detection of intracellular nitric oxide (NO). Once inside the cell, it is hydrolyzed by cytosolic esterases to cell-impermeant DAF-FM. DAF-FM reacts with NO+ equivalents, such as nitric anhydride (N2O3), which are formed by autoxidation of NO. Under aerobic conditions, DAF-FM can rapidly and irreversibly trap NO to yield highly fluorescent triazolofluoresceins (DAF-Ts). DAF-FM DA is photostable and stable in pH 5.5 to 12. It can be used to quantitatively monitor agonist-evoked NO synthesis in cultured HUVECs. DAF-FM DA may also be used to quantify NO levels in induced pluripotent stem cell-derived derived cardiomyocytes (iPSC-CM).It is recommended to prepare stock solutions in DMSO.
Optical Data
| Emission Color | Green |
| λabs | 495 nm |
| λem | 515 nm |
| Extinction Coefficient (ε) | 84000 M-1cm-1 |
| Cell Permeable | Yes |
| pH Sensitivity | Independent of pH when pH >5.8 |
| Application | Confocal Microscopy, Flow cytometry |
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Product Specifications
Molecular Weight
496.42
Formula
C25H18F2N2O7
Storage
Store at -20°C
Purity
≥80% (HPLC)
Chemical Name
4-Amino-5-methylamino-2',7'-difluorofluorescein diacetate
CAS Number
254109-22-3
PubChem ID
2762646
InChI Key
BEVHTVRRVVEMEF-UHFFFAOYSA-N
SMILES
O=C1OC2(C3=CC=C(C(N)=C13)NC)C4=CC(F)=C(C=C4OC5=CC(OC(C)=O)=C(C=C52)F)OC(C)=O
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
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Background References
References are publications that support the biological activity of the product.
- Xu Uncompensated mitochondrial oxidative stress underlies heart failure in an iPSC-derived model of congenital heart disease. Cell Stem Cell 2022 PMID: 35395180
- Sheng DAF-FM (4-amino-5-methylamino-2',7'-difluorofluorescein) diacetate detects impairment of agonist-stimulated nitric oxide synthesis by elevated glucose in human vascular endothelial cells: reversal by vitamin C and L-sepiapterin. J.Pharmacol.Exp.Ther. 2005 PMID: 16093274
- Almeida Sensing nitric oxide in cells: historical technologies and future outlook. ACS.Sens. 2021 PMID: 33871990
- Kojima Fluorescent Indicators for Imaging Nitric Oxide Production. Angew.Chem.Int.Ed.Engl. 1999 PMID: 10556905
Product Specific Notices for DAF FM diacetate
For research use only
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