DAF FM diacetate
Tocris Bioscience, part of Bio-Techne | Catalog # 7756
Key Product Details
| Description: | Cell permeable photostable nitric oxide (NO) fluorescent indicator |
| Chemical Name: | 4-Amino-5-methylamino-2',7'-difluorofluorescein diacetate |
| Purity: | ≥80% (HPLC) |
| Molecular Weight: | 496.42 |
Biological Activity for DAF FM diacetate
DAF-FM DA is a cell-permeable fluorescent probe for the detection of intracellular nitric oxide (NO). Once inside the cell, it is hydrolyzed by cytosolic esterases to cell-impermeant DAF-FM. DAF-FM reacts with NO+ equivalents, such as nitric anhydride (N2O3), which are formed by autoxidation of NO. Under aerobic conditions, DAF-FM can rapidly and irreversibly trap NO to yield highly fluorescent triazolofluoresceins (DAF-Ts). DAF-FM DA is photostable and stable in pH 5.5 to 12. It can be used to quantitatively monitor agonist-evoked NO synthesis in cultured HUVECs. DAF-FM DA may also be used to quantify NO levels in induced pluripotent stem cell-derived derived cardiomyocytes (iPSC-CM).
It is recommended to prepare stock solutions in DMSO.
Optical Data for DAF FM diacetate
| Emission Color | Green |
|---|---|
| λabs | 495 nm |
| λem | 515 nm |
| Extinction Coefficient (ε) | 84000 M-1cm-1 |
| Cell Permeable | Yes |
| pH Sensitivity | Independent of pH when pH >5.8 |
| Application | Confocal Microscopy, Flow cytometry |
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