DAF FM diacetate

Name: DAF FM diacetate
Brand: Tocris Bioscience
SKU: 7756

Catalog # 7756 | Tocris Bioscience a Bio-Techne Brand

COA / SDS

Cell permeable photostable nitric oxide (NO) fluorescent indicator

Key Product Details

Description:	Cell permeable photostable nitric oxide (NO) fluorescent indicator
Chemical Name:	4-Amino-5-methylamino-2',7'-difluorofluorescein diacetate
Purity:	≥80% (HPLC)
Molecular Weight:	496.42

View Full Technical Details for DAF FM diacetate

Technical Data
Citations / Reviews

Biological Activity for DAF FM diacetate

DAF-FM DA is a cell-permeable fluorescent probe for the detection of intracellular nitric oxide (NO). Once inside the cell, it is hydrolyzed by cytosolic esterases to cell-impermeant DAF-FM. DAF-FM reacts with NO⁺ equivalents, such as nitric anhydride (N₂O₃), which are formed by autoxidation of NO. Under aerobic conditions, DAF-FM can rapidly and irreversibly trap NO to yield highly fluorescent triazolofluoresceins (DAF-Ts). DAF-FM DA is photostable and stable in pH 5.5 to 12. It can be used to quantitatively monitor agonist-evoked NO synthesis in cultured HUVECs. DAF-FM DA may also be used to quantify NO levels in induced pluripotent stem cell-derived derived cardiomyocytes (iPSC-CM).

It is recommended to prepare stock solutions in DMSO.

Optical Data for DAF FM diacetate

Emission Color	Green
λ_abs	495 nm
λ_em	515 nm
Extinction Coefficient (ε)	84000 M^-1cm^-1
Cell Permeable	Yes
pH Sensitivity	Independent of pH when pH >5.8
Application	Confocal Microscopy, Flow cytometry

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Technical Data for DAF FM diacetate

M. Wt	496.42
Formula	C₂₅H₁₈F₂N₂O₇
Storage	Store at -20°C
Purity	≥80% (HPLC)
CAS Number	254109-22-3
PubChem ID	2762646
InChi Key	BEVHTVRRVVEMEF-UHFFFAOYSA-N
Smiles	O=C1OC2(C3=CC=C(C(N)=C13)NC)C4=CC(F)=C(C=C4OC5=CC(OC(C)=O)=C(C=C52)F)OC(C)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

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References for DAF FM diacetate

References are publications that support the biological activity of the product.

Xu Uncompensated mitochondrial oxidative stress underlies heart failure in an iPSC-derived model of congenital heart disease. Cell Stem Cell 2022 PMID: 35395180
Almeida Sensing nitric oxide in cells: historical technologies and future outlook. ACS.Sens. 2021 PMID: 33871990
Sheng DAF-FM (4-amino-5-methylamino-2',7'-difluorofluorescein) diacetate detects impairment of agonist-stimulated nitric oxide synthesis by elevated glucose in human vascular endothelial cells: reversal by vitamin C and L-sepiapterin. J.Pharmacol.Exp.Ther. 2005 PMID: 16093274
Kojima Fluorescent Indicators for Imaging Nitric Oxide Production. Angew.Chem.Int.Ed.Engl. 1999 PMID: 10556905

Citations for DAF FM diacetate

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