D-Luciferin sodium salt
Tocris Bioscience, part of Bio-Techne | Catalog # 5427
Firefly luciferase substrate; cell permeable.
Key Product Details
Description
Firefly luciferase substrate; cell permeable.
Product Description
D-Luciferin sodium salt is a firefly luciferase substrate (Km = ~2 μM). Used in many standard bioluminescence imaging (BLI) techniques, including monitoring tumour growth, and investigating intracellular signaling activity in vitro and in vivo. Used with MC3 LNP Luciferase (Cat. No. 8889) for in vitro and in vivo bioluminescence LNP detection.Cell permeable. Emission maximum = 562 nm.Optical Data
| Emission Color | Green |
| λem | 562 nm |
| Cell Permeable | Yes |
| Application | Bioluminescence Imaging (BLI) Techniques, including monitoring tumor growth |
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Product Specifications
Molecular Weight
302.30
Formula
C11H7N2NaO3S2
Storage
Store at -20°C
Purity
≥98% (HPLC)
Chemical Name
(4S)-4,5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylic acid sodium salt
CAS Number
103404-75-7
PubChem ID
23710675
InChI Key
BZNVUYVALNTPBG-SREJTOIWSA-M
SMILES
OC1=CC2=C(N=C(C3=N[C@@H]([C@@]([O-])=O)CS3)S2)C=C1.[Na+]
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| water | 30.23 | 100 | |
| DMSO | 30.23 | 100 |
Preparing Stock Solutions
for D-Luciferin sodium salt
The following data is based on the product molecular weight 302.30
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 3.31 mL | 16.54 mL | 33.08 mL |
| 5 mM | 0.66 mL | 3.31 mL | 6.62 mL |
| 10 mM | 0.33 mL | 1.65 mL | 3.31 mL |
| 50 mM | 0.07 mL | 0.33 mL | 0.66 mL |
Calculators
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Background References
References are publications that support the biological activity of the product.
- Keyaerts Dynamic bioluminescence imaging for quantitative tumour burden assessment using IV or IP administration of D: -luciferin: effect on intensity, time kinetics and repeatability of photon emission. Eur.J.Nucl.Med.Mol.Imaging. 2008 PMID: 18180921
Product Specific Notices for D-Luciferin sodium salt
For research use only
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