Cyanine 5 Tyramide
Tocris Bioscience, part of Bio-Techne | Catalog # 6458
Red fluorescent reagent widely used for signal amplification in IHC and FISH
Key Product Details
Description
Red fluorescent reagent widely used for signal amplification in IHC and FISH
Alternative Names
Cy5 Tyramide
Product Description
Cyanine 5 Tyramide is a red fluorescent reagent widely used for tyramide signal amplification (TSA) in IHC, ICC, FISH and multicolor FISH. HRP catalyzes localized deposition of multiple tyramide molecules (catalyzed reporter deposition, CARD), binding the fluorescein tyramide to adjacent tyrosines to enhance fluorescent signal.Optical Data
| Emission Color | Red |
| λabs | 651 nm |
| λem | 665 nm |
| Extinction Coefficient (ε) | 250000 M-1cm-1 |
| Closest Laser line | 647 nm |
| Application | Tyramide Signal Amplification (TSA) in IHC, ICC, ISH, FISH |
Spectra Viewer
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Product Specifications
Molecular Weight
775.97
Formula
C41H49N3O8S2
Storage
Store at -20°C
Purity
≥95% (HPLC)
Chemical Name
2-[5-[1,3-Dihydro-1-[6-[[2-(4-hydroxyphenyl)ethyl]amino]-6-oxohexyl]3,3-dimethyl-5-sulfo-2H-indol-2-ylidene]-1,3-pentadien-1-yl]-1-ethyl-3,3-dimethyl-5-sulfo-3H-indolium inner salt
CAS Number
1431148-26-3
PubChem ID
135290897
InChI Key
NHSJCIBSNKLAMA-UHFFFAOYSA-N
SMILES
CC[N+]1=C(C(C)(C2=CC(S([O-])(=O)=O)=CC=C12)C)/C=C/C=C/C=C3N(C4=CC=C(S(O)(=O)=O)C=C4C/3(C)C)CCCCCC(NCCC5=CC=C(C=C5)O)=O
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 11.64 | 15 |
Preparing Stock Solutions
for Cyanine 5 Tyramide
The following data is based on the product molecular weight 775.97
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.15 mM | 8.59 mL | 42.96 mL | 85.91 mL |
| 0.75 mM | 1.72 mL | 8.59 mL | 17.18 mL |
| 1.5 mM | 0.86 mL | 4.30 mL | 8.59 mL |
| 7.5 mM | 0.17 mL | 0.86 mL | 1.72 mL |
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Background References
References are publications that support the biological activity of the product.
- Matsumoto Aldehyde dehydrogenase 1B1: a novel immunohistological marker for colorectal cancer. Br.J.Cancer 2017 PMID: 28881356
- Wong Identification of differentially expressed genes from ovarian cancer cells by MICROMAX cDNA microarray system. Biotechniques 2001 PMID: 11252802
- Chanda Identification of the SUMO E3 ligase PIAS1 as a potential survival biomarker in breast cancer. PLoS One 2017 PMID: 28493978
Product Specific Notices for Cyanine 5 Tyramide
For research use only
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