BromoCatch™ Ligand, Alkyne

Name: BromoCatch™ Ligand, Alkyne
Brand: Tocris Bioscience
SKU: 8940

Tocris Bioscience, part of Bio-Techne | Catalog # 8940

COA / SDS

Clickable alkyne-functionalized covalent probe for BromoCatch-tagged proteins

Key Product Details

Description:	Clickable alkyne-functionalized covalent probe for BromoCatch-tagged proteins
Chemical Name:	(2R)-2-((6S)-4-(4-Acrylamidophenyl)-2,3,9-trimethyl-6H-thieno[3,2-f][1,2,4]triazolo[4,3-a][1,4]diazepin-6-yl)-N-(2-(2-(2-(prop-2-yn-1-yloxy)ethoxy)ethoxy)ethyl)butanamide
Purity:	≥95% (HPLC)
Molecular Weight:	632.78

View Full Technical Details for BromoCatch™ Ligand, Alkyne

Technical Data
Citations

Biological Activity for BromoCatch™ Ligand, Alkyne

BromoCatch™ Ligand, Alkyne is a covalent ligand engineered to irreversibly bind BromoCatch-tagged proteins via selective reaction with the E438C cysteine mutant of the Brd4-BD2 domain. Featuring a terminal alkyne appended to the BromoCatch Ligand scaffold, this ligand enables downstream modification through copper-catalyzed azide-alkyne cycloaddition (CuAAC). BromoCatch™ Ligand, Alkyne facilitates modular tagging of BromoCatch fusions with fluorophores, biotin, affinity tags, or functional moieties, offering a flexible platform for custom probe development. It enables site-specific protein labeling in vitro and is ideal for applications in chemical biology, proteomics, and multiplexed detection assays.

Please refer to this protocol for further information on how to use this product.

This product is part of an early adopter program.

BromoCatch™ is a trademark of Bio-Techne Corp.

Licensing Information

Sold under exclusive licence from the University of Dundee

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Technical Data for BromoCatch™ Ligand, Alkyne

M. Wt	632.78
Formula	C₃₃H₄₀N₆O₅S
Storage	Store at -20°C
Purity	≥95% (HPLC)
InChi Key	OFMNYRGCADESPS-VIZCGCQYSA-N
Smiles	CC1=C(C)SC2=C1C(C3=CC=C(C=C3)NC(C=C)=O)=N[C@@H]([C@H](C(NCCOCCOCCOCC#C)=O)CC)C4=NN=C(C)N24

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

Mass

Concentration

Volume

M. Wt*

g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

References for BromoCatch™ Ligand, Alkyne

References are publications that support the biological activity of the product.

Rodriguez-Rios BromoCatch: a self-labelling tag platform for protein analysis and live cell imaging. biorxiv