BL660-NO
Tocris Bioscience, part of Bio-Techne | Catalog # 7753
Nitric oxide activity-based sensing NIR bioluminescent probe
Key Product Details
Description
Nitric oxide activity-based sensing NIR bioluminescent probe
Product Description
BL660-NO is an activity-based sensing (ABS) bioluminescent probe for near-infrared red (NIR) detection of nitric oxide (NO). BL660-NO is suitable for in vitro, live cell and deep tissue imaging. Used to visualize endogenous NO in vitro, and in murine models of liver and breast cancer. Used to investigate the effect of diet on tumor microenvironment (TME) in mice.This product is a scalemic mixture of (S) and (R) enantiomers in which (S) is predominant. The exact ratio is batch specific and is expressed as an enantiomeric excess (e.e.) on the Certificate of Analysis.
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Product Specifications
Molecular Weight
420.58
Formula
C24H28N4OS
Storage
Store at -20°C
Purity
≥90% (HPLC)
Chemical Name
N-(2-Aminophenyl)-2-((1E,3E)-4-(4-(diethylamino)phenyl)buta-1,3-dien-1-yl)-4,5-dihydrothiazole-4-carboxamide
InChI Key
LERSAEPSTKIDTF-PIHCAMFYSA-N
SMILES
O=C(NC1=C(N)C=CC=C1)C(CS2)N=C2/C=C/C=C/C(C=C3)=CC=C3N(CC)CC
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 42.06 | 100 |
Preparing Stock Solutions
for BL660-NO
The following data is based on the product molecular weight 420.58
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.38 mL | 11.89 mL | 23.78 mL |
| 5 mM | 0.48 mL | 2.38 mL | 4.76 mL |
| 10 mM | 0.24 mL | 1.19 mL | 2.38 mL |
| 50 mM | 0.05 mL | 0.24 mL | 0.48 mL |
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Background References
References are publications that support the biological activity of the product.
- Yadav Activity-based NIR bioluminescence probe enables discovery of diet-induced modulation of the tumor microenvironment via nitric oxide. ACS Cent.Sci. 2022 PMID: 35505872
Product Specific Notices for BL660-NO
For research use only
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