Biotinyl Tyramide
Tocris Bioscience, part of Bio-Techne | Catalog # 6241
Reagent widely used for signal amplification in IHC and FISH
Key Product Details
Description
Reagent widely used for signal amplification in IHC and FISH
Product Description
Biotinyl Tyramide is a reagent widely used for signal amplification in IHC and fluorescent in situ hybridization (ISH) and APEX proximity labeling. HRP catalyzes localized deposition of multiple biotinyl tyramide molecules (catalyzed reporter deposition, CARD), which can be detected using a labeled streptavidin conjugate. The localization of deposition around HRP leads to good resolution. Suitable for either fluorescence or chromogenic detection. Biotinyl Tyramide is used as a substrate for APEX proximity labeling.Spectra Viewer
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Product Specifications
Molecular Weight
363.47
Formula
C18H25N3O3S
Storage
Store at -20°C
Purity
≥98% (HPLC)
Chemical Name
(3aS,4S,6aR)-Hexahydro-N-[2-(4-hydroxyphenyl)ethyl]-2-oxo-1H-thieno[3,4-d]imidazole-4-pentanamide
CAS Number
41994-02-9
PubChem ID
21826423
InChI Key
VZWXNOBHWODXCW-ZOBUZTSGSA-N
SMILES
[H][C@]12CS[C@H]([C@]1(NC(N2)=O)[H])CCCCC(NCCC3=CC=C(C=C3)O)=O
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 36.35 | 100 |
Preparing Stock Solutions
for Biotinyl Tyramide
The following data is based on the product molecular weight 363.47
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.75 mL | 13.76 mL | 27.51 mL |
| 5 mM | 0.55 mL | 2.75 mL | 5.50 mL |
| 10 mM | 0.28 mL | 1.38 mL | 2.75 mL |
| 50 mM | 0.06 mL | 0.28 mL | 0.55 mL |
Calculators
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Background References
References are publications that support the biological activity of the product.
- McKay Amplification of fluorescent in situ hybridisation signals in formalin fixed paraffin wax embedded sections of colon tumour using biotinylated tyramide. Mol.Pathol. 1997 PMID: 9536283
- Bobrow Catalyzed reporter deposition, a novel method of signal amplification. Application to immunoassays. J.Immunol.Methods 1989 PMID: 2558138
Product Specific Notices for Biotinyl Tyramide
For research use only
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