BDY FL-X, SE
Tocris Bioscience, part of Bio-Techne | Catalog # 5620
Key Product Details
| Description: | Green BDY (BODIPY® or boron-dipyrromethene) fluorescent dye for the labeling of amines; supplied as NHS ester |
| Chemical Name: | (T-4)-[5-[(3,5-Dimethyl-2H-pyrrol-2-ylidene-&N)methyl]-[N-[6-[(2,5-Dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]-1H-pyrrole-2-propanamidato-κN1]difluoroboron |
| Purity: | ≥90% (HPLC) |
| Molecular Weight: | 502.32 |
Biological Activity for BDY FL-X, SE
BDY FL-X, SE is a fluorescent green BDY (BODIPY® or boron-dipyrromethene) dye for the labeling of amines. Exhibits similar excitation and emission spectra to FITC and TFAX 488 dyes. Displays high fluorescence quantum yield and high extinction coefficient and is relatively insensitive to pH change. This dye contains a C3 alkyl spacer between the fluorophore and the NHS ester group, reducing the potential for interactions between fluorophore and conjugated biomolecule. The hydrophobic nature of BDY FL makes it ideal for labeling lipids and cell membranes. BDY FL also exhibits narrow emission bandwidths and has a relatively long excited-state lifetime, making it ideal for fluorescence polarization assays and two-photon excitation (TPE) microscopy. Excitation maximum ~504 nM; emission maximum ~510 nM.
BODIPY® is a registered trademark of Molecular Probes.
Optical Data for BDY FL-X, SE
| Emission Color | Green |
|---|---|
| λabs | 504 nm |
| λem | 510 nm |
| Extinction Coefficient (ε) | 85000 M-1cm-1 |
| Reactive Group | NHS ester |
| Reactivity | Primary amines |
| Cell Permeable | No |
| Application | Confocal fluorescence p, fluorescence polarization assays and two-photon excitation (TPE) microscopy |
| Alternative For | FAM; Alexa Fluor 488; DyLight 488; Cy2 |
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.
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