TFAX 488, SE
Tocris Bioscience, part of Bio-Techne | Catalog # 6619
Key Product Details
| Description: | Green fluorescent dye; amine reactive |
| Chemical Name: | 3,6-Diamino-9-[2-carboxy-4(5)-[[(2,5-dioxo-1-pyrrolidinyl)oxy]carbonyl]phenyl]-4,5-disulfoxanthylium bislithium salt |
| Molecular Weight: | 643.41 |
Biological Activity for TFAX 488, SE
TFAX 488, SE is an amine reactive green fluorescent dye. Insensitive to pH in the range 4 - 10. Forms bright and photostable conjugates with proteins and antibodies. Suitable for use in flow cytometry, two-photon excitation microscopy (TPE), and super resolution microscopy techniques, such as dSTORM, SIM and STED. Excitation maximum = 495 nm; emission maximum = 515 nm; extinction coefficient = 73,000 M-1cm-1; quantum yield = 0.92.
Optical Data for TFAX 488, SE
| Emission Color | Green |
|---|---|
| Brightness | |
| λabs | 495 nm |
| λem | 515 nm |
| Extinction Coefficient (ε) | 73,000 M-1cm-1 |
| Quantum Yield (φ) | 0.92 |
| Closest Laser line | 488 nm |
| Reactive Group | NHS ester |
| Reactivity | Primary amines |
| Cell Permeable | No |
| pH Sensitivity | pH insensitive pH 4-10 |
| Application | flow cytometry, two-photon excitation microscopy, dSTORM, SIM, STED |
| Alternative For | Alexa Fluor® 488, AF 488, FITC, Cy2 |
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.
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