TFAX 488, SE
Tocris Bioscience, part of Bio-Techne | Catalog # 6619
Green fluorescent dye; amine reactive
Discontinued Product
6619 has been discontinued.
View all TFAX Dyes products.
Key Product Details
Description
Green fluorescent dye; amine reactive
Product Description
TFAX 488, SE is an amine reactive green fluorescent dye. Insensitive to pH in the range 4 - 10. Forms bright and photostable conjugates with proteins and antibodies. Suitable for use in flow cytometry, two-photon excitation microscopy (TPE), and super resolution microscopy techniques, such as dSTORM, SIM and STED. Excitation maximum = 495 nm; emission maximum = 515 nm; extinction coefficient = 73,000 M-1cm-1; quantum yield = 0.92.Optical Data
| Emission Color | Green |
| Brightness | |
| λabs | 495 nm |
| λem | 515 nm |
| Extinction Coefficient (ε) | 73,000 M-1cm-1 |
| Quantum Yield (φ) | 0.92 |
| Closest Laser line | 488 nm |
| Reactive Group | NHS ester |
| Reactivity | Primary amines |
| Cell Permeable | No |
| pH Sensitivity | pH insensitive pH 4-10 |
| Application | flow cytometry, two-photon excitation microscopy, dSTORM, SIM, STED |
| Alternative For | Alexa Fluor® 488 , AF 488, FITC, Cy2 |
Trademarks and registered trademarks are the property of their respective owners.
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectra Viewer
Product Specifications
Molecular Weight
643.41
Formula
C25H15N3O13S2Li2
Storage
Store at -20°C
Chemical Name
3,6-Diamino-9-[2-carboxy-4(5)-[[(2,5-dioxo-1-pyrrolidinyl)oxy]carbonyl]phenyl]-4,5-disulfoxanthylium bislithium salt
CAS Number
222164-96-7
PubChem ID
23543054
InChI Key
FYCGGEVYPRTFDS-UHFFFAOYSA-L
SMILES
[NH2+]=C1C=CC2=C(C3=CC=C(C(ON4C(CCC4=O)=O)=O)C=C3C([O-])=O)C5=CC=C(N)C(S(=O)([O-])=O)=C5OC2=C1S(=O)([O-])=O
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Panchuk-Voloshina Alexa dyes, a series of new fluorescent dyes that yield exceptionally bright, photostable conjugates. J.Histochem.Cytochem. 1999 PMID: 10449539
- Iwata Increased susceptibility of cytoplasmic over nuclear polyglutamine aggregates to autophagic degradation. Proc.Natl.Acad.Sci.USA. 2005 PMID: 16141322
- Burnette Bleaching/blinking assisted localization microscopy for superresolution imaging using standard fluorescent molecules. Proc.Natl.Acad.Sci.USA. 2011 PMID: 22167805
- van de Linde Direct stochastic optical reconstruction microscopy with standard fluorescent probes. Nat.Protoc. 2011 PMID: 21720313
- Rossi Super-resolution imaging of aquaporin-4 orthogonal arrays of particles in cell membranes. J.Cell Sci. 2012 PMID: 22718347
Product Specific Notices for TFAX 488, SE
For research use only
Loading...
Loading...