BDY FL, SE
Tocris Bioscience, part of Bio-Techne | Catalog # 5465
Green BDY (BODIPY® or boron-dipyrromethene) fluorescent dye supplied with an NHS ester for the labeling of primary amines
Key Product Details
Description
Green BDY (BODIPY® or boron-dipyrromethene) fluorescent dye supplied with an NHS ester for the labeling of primary amines
Product Description
Key information: BDY FL, SE is a fluorescent green BDY (BODIPY® or boron-dipyrromethene) dye supplied with an NHS ester reactive group for the labeling of primary amines. The hydrophobic nature of BDY FL makes it ideal for labeling lipids and cell membranes.Application: BDY FL, SE exhibits a narrow emission bandwidth and has a relatively long excited-state lifetime, making it ideal for fluorescence polarization assays and two-photon excitation (TPE) microscopy.
Properties and Photophysical Data: Displays high fluorescence quantum yield and high extinction coefficient and is relatively insensitive to pH change. This dye contains a C3 alkyl spacer between the fluorophore and the NHS ester group, reducing the potential for interactions between fluorophore and conjugated biomolecule. Exhibits similar excitation and emission spectra to dye FITC. Excitation and emission maxima (λ) are 502 nM and 510 nM, respectively; quantum yield = 0.97; extinction coefficient = 80,000 M-1cm-1 (measured in TFE plus 0.1% TFA); A280 = 0.027.
For more information on how BDY FL, SE may be used, see our protocol: Conjugation Protocol for Amine Reactive Dyes.
It is recommended to prepare stock solutions in DMSO.
BODIPY® is a registered trademark of Molecular Probes.
Optical Data
| Emission Color | Green |
| Brightness | |
| λabs | 502 nm |
| λem | 510 nm |
| Extinction Coefficient (ε) | 80000 M-1cm-1 |
| Quantum Yield (φ) | 0.97 |
| Closest Laser line | 488 nm |
| Reactive Group | NHS ester |
| Reactivity | Primary amines |
| Cell Permeable | No |
| Correction Factor 260 | 0.015 |
| Correction Factor 280 | 0.027 |
| Application | Confocal Microscopy, Fluorescence Polarization Assays and Two-Photon Excitation (TPE) Microscopy |
| Alternative For | FAM; Alexa Fluor® 488; DyLight™ 488; Cy2 |
Trademarks and registered trademarks are the property of their respective owners.
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Product Specifications
Molecular Weight
389.16
Formula
C18H18BF2N3O4
Storage
Store at -20°C
Purity
≥95% (HPLC)
Chemical Name
(T-4)-[1-[3-[5-[(3,5-Dimethyl-2H-pyrrol-2-ylidene-κN)methyl]-1H-pyrrol-2-yl-κN]-1-oxopropoxy]-2,5-pyrrolidinedionato]difluoroboron
CAS Number
146616-66-2
PubChem ID
15410442
InChI Key
SDVMGNSTOAJONW-UHFFFAOYSA-N
SMILES
CC(C=C1C)=C2N1[B-](F)(F)[N+]3=C(CCC(ON4C(CCC4=O)=O)=O)C=CC3=C2
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
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Background References
References are publications that support the biological activity of the product.
- Kubohara Mitochondria are the target organelle of differentiation-inducing factor-3, an anti-tumor agent isolated from Dictyostelium discoideum. PLoS ONE 2013 PMID: 23977224
Product Specific Notices for BDY FL, SE
For research use only
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