Acridine Orange hydrochloride

Name: Acridine Orange hydrochloride
Brand: Tocris Bioscience
SKU: 5092

Catalog # 5092 | Tocris Bioscience a Bio-Techne Brand

Citations (1)

COA / SDS

Nucleic acid binding dye

Key Product Details

Description:	Nucleic acid binding dye
Chemical Name:	N,N,N',N'-Tetramethyl-3,6-acridinediamine hydrochloride
Purity:	≥98% (HPLC)

View Full Technical Details for Acridine Orange hydrochloride

Technical Data
Citations (1) / Reviews

Biological Activity for Acridine Orange hydrochloride

Acridine Orange hydrochloride is a cell and organelle membrane permeable nucleic acid binding dye. Emits green fluorescence when bound to double-stranded DNA and red fluorescence when bound to RNA or single-stranded DNA. Used in cell cycle and apoptosis studies and as a lysosomal dye.

This product is supplied as a hydrochloride salt, the stoichiometry of which is batch dependent. Please refer to the Certificate of Analysis to obtain the batch specific Net Product Content.

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Technical Data for Acridine Orange hydrochloride

Formula	C₁₇H₁₉N₃.HCl
Storage	Store at RT
Purity	≥98% (HPLC)
PubChem ID	517204
InChi Key	VSTHNGLPHBTRMB-UHFFFAOYSA-N
Smiles	CN(C)C1=CC=C2C(N=C(C=C(N(C)C)C=C3)C3=C2)=C1.Cl
M. Wt	265.36 (Free Base)

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Acridine Orange hydrochloride

Solubility

Soluble to 10mg/ml in water

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References for Acridine Orange hydrochloride

References are publications that support the biological activity of the product.

Kiyoshima Chemoresistance to concanamycin A1 in human oral squamous cell carcinoma is attenuated by an HDAC inhibitor partly via suppression of Bcl-2 expression. PLoS ONE 2013 PMID: 24278362
Ratan Rapid communication: oxidative stress induces apoptosis in embryonic cortical neurons. J.Neurobiol. 2008 PMID: 7903353
McMaster Analysis of single and double stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange. Proc.Natl.Acad.Sci.USA 1977 PMID: 73185

Citations for Acridine Orange hydrochloride (1)

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Citations are publications that use Bio-Techne products. Selected citations for Acridine Orange hydrochloride include:

Stephan et al. (2022) Imatinib disturbs lysosomal function and morphology and impairs the activity of mTORC1 in human hepatocyte cell lines. Food Chem Toxicol. 162:112869
PMID: 35182693

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