Mouse Mesenchymal Stem Cell Multi-Color Flow Kit
Catalog # FMC003 | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Assay Procedure
Refer to the product datasheet for complete product details.
Reagents Provided
Reagents Supplied in the Mouse Mesenchymal Stromal Cell 4-Color Flow Cytometry Kit (Catalog # FMC003):
- Flow Cytometry Staining Buffer (100 mL)
Positive Markers
- Rat IgG2A Anti-Mouse Endoglin/CD105-Fluorescein (Clone 209701)
- Rat IgG2A Anti-Mouse Integrin beta 1/CD29-PE (Clone 265917)
- Rat IgG2A Anti-Mouse Sca-1-APC (Clone 177228)
Negative Markers
- Rat IgG2B Anti-Mouse CD45-PerCP (Clone 30-F11)
Other Supplies Required
Reagents
- Fc Receptor Blocking Reagents
Materials
- Flow Cytometry Tubes
Equipment
- Clinical Centrifuge
Procedure Overview






- Wash the cells with Flow Cytometry Staining Buffer.
Perform a cell count. - Add Fc receptor blocking reagents. If using excess pre-immune IgG to block Fc receptor, the excess IgG does not need to be washed from the cells following the incubation period.
- Transfer approximately 100 µL of the Fc receptor-blocked cells (about 1 x 106 cells) into a 5 mL flow cytometry tube.
- Add 10 µL of each fluorochrome-conjugated antibody or each corresponding isotype control antibody.
- Incubate the mixture for 30-45 minutes at room temperature in the dark.
- Wash the cells with 2 mL Flow Cytometry Staining Buffer.
- Resuspend the cell pellet in 200-400 µL of Flow Cytometry Staining Buffer.
- Analyze expression of MSC markers simultaneously by flow cytometry.
Citations for Mouse Mesenchymal Stem Cell Multi-Color Flow Kit (5)
Citations are publications that use Bio-Techne products. Selected citations for Mouse Mesenchymal Stem Cell Multi-Color Flow Kit include:
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Species: Mouse
Sample Types: Whole Cells
QT Bui et al. (2023-01-10), Disruption of CCL2 in Mesenchymal Stem Cells as an Anti-Tumor Approach against Prostate Cancer Cancers, 2023-015(2).
PMID: 36672395 -
Species: Mouse
Sample Types: Whole Cells
Applications: Flow CytometryT Hirakawa et al. (2022-05-16), Trophic and immunomodulatory effects of adipose tissue derived stem cells in a preclinical murine model of endometriosis Scientific Reports, 2022-012(1):8031.
PMID: 35577867 -
Species: Mouse
Sample Types: Whole Cells
Applications: Flow CytometryJ Su et al. (2020-07-06), Cellular senescence mediates the detrimental effect of prenatal dexamethasone exposure on postnatal long bone growth in mouse offspring Stem Cell Res Ther, 2020-011(1):270.
PMID: 32631432 -
Species: Mouse
Sample Types: Whole Cells
Y Hu et al. (2020-05-19), Defactinib attenuates osteoarthritis by inhibiting positive feedback loop between H-type vessels and MSCs in subchondral bone J Orthop Translat, 2020-024(0):12-22.
PMID: 32518750 -
Species: Mouse
Applications: Flow CytometryCC Shu et al. (2020-02-14), The relationship between synovial inflammation, structural pathology, and pain in post-traumatic osteoarthritis: differential effect of stem cell and hyaluronan treatment Arthritis Res. Ther., 2020-022(1):29.
PMID: 32059749
There are no citations that match your criteria.
FAQs for Mouse Mesenchymal Stem Cell Multi-Color Flow Kit
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Do reagents in Mouse Mesenchymal Stem Cell Multi-Color Flow Kit (Catalog # FMC003) cross -react with Human MSCs?
The antibodies in the Mouse Mesenchymal Stem Cell Multi-Color Flow Kit are specific to mouse MSCs. We have separate Human Mesenchymal Stem Cell Multi-Color flow Kits, Catalog # FMC020 or FMC002 that contain antibodies for human markers.
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Will the Human Mesenchymal Stem Cell Multi-Color Flow Kit (catalog # FMC002) or Mouse Mesenchymal Stem Cell Multi-Color Flow Kit (catalog # FMC003) work for identificaiton of Rat Menchymal stem cells?
The Human Mesenchymal Stem Cell Multi-Color Flow Kit (catalog # FMC002) or Mouse Mesenchymal Stem Cell Multi-Color Flow Kit (catalog # FMC003) will not work for identificaiton of Rat Mesenchymal stem cells.