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Mouse Mesenchymal Stem Cell Multi-Color Flow Kit

Key Product Details

Verification of Mouse Mesenchymal Stem/Stromal Cell Identity by Analysis of Mesenchymal Stem Cell Marker Expression.
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FMC003

Assay Procedure

Refer to the product datasheet for complete product details.

Briefly, mouse mesenchymal stem/stromal cell marker expression can be assessed using the following procedure:

  • Incubate the cells with the provided antibodies
  • Wash the cells
  • Resuspend the cells in Flow Cytometry Staining Buffer
  • Analyze the samples by flow cytometry
 
 

Reagents Provided

Reagents Supplied in the Mouse Mesenchymal Stromal Cell 4-Color Flow Cytometry Kit (Catalog # FMC003):

  • Flow Cytometry Staining Buffer (100 mL)

Positive Markers

  • Rat IgG2A Anti-Mouse Endoglin/CD105-Fluorescein (Clone 209701)
  • Rat IgG2A Anti-Mouse Integrin beta 1/CD29-PE (Clone 265917)
  • Rat IgG2A Anti-Mouse Sca-1-APC (Clone 177228)

Negative Markers

  • Rat IgG2B Anti-Mouse CD45-PerCP (Clone 30-F11)

Other Supplies Required

Reagents

  • Fc Receptor Blocking Reagents

Materials

  • Flow Cytometry Tubes

Equipment

  • Clinical Centrifuge

Procedure Overview

 

 

 

 

 

 
  1. Wash the cells with Flow Cytometry Staining Buffer.
    Perform a cell count.
  2. Add Fc receptor blocking reagents. If using excess pre-immune IgG to block Fc receptor, the excess IgG does not need to be washed from the cells following the incubation period.
  3. Transfer approximately 100 µL of the Fc receptor-blocked cells (about 1 x 106 cells) into a 5 mL flow cytometry tube.
  4. Add 10 µL of each fluorochrome-conjugated antibody or each corresponding isotype control antibody.
  5. Incubate the mixture for 30-45 minutes at room temperature in the dark.
  6. Wash the cells with 2 mL Flow Cytometry Staining Buffer.
  7. Resuspend the cell pellet in 200-400 µL of Flow Cytometry Staining Buffer.
  8. Analyze expression of MSC markers simultaneously by flow cytometry.

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FAQs

  • Do reagents in Mouse Mesenchymal Stem Cell Multi-Color Flow Kit (Catalog # FMC003) cross -react with Human MSCs?

    The antibodies in the Mouse Mesenchymal Stem Cell Multi-Color Flow Kit are specific to mouse MSCs.  We have separate Human Mesenchymal Stem Cell Multi-Color flow Kits, Catalog # FMC020 or FMC002 that contain antibodies for human markers.

  • Will  the Human Mesenchymal Stem Cell Multi-Color Flow Kit (catalog # FMC002) or Mouse Mesenchymal Stem Cell Multi-Color Flow Kit (catalog # FMC003) work for identificaiton of Rat Menchymal stem cells?

    The Human Mesenchymal Stem Cell Multi-Color Flow Kit (catalog # FMC002) or Mouse Mesenchymal Stem Cell Multi-Color Flow Kit (catalog # FMC003) will not work for identificaiton of Rat Mesenchymal stem cells.

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

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