Human Mesenchymal Stem Cell Verification Flow Kit

Kit Summary

Antibodies for MSC verification according to the markers proposed by the International Society for Cellular Therapy.

Why use the ISCT-recommended Markers to Verify MSC Identity?

The different methods used to isolate, culture, differentiate, and define mesenchymal stem cells (MSCs) can lead to experimental variability and contradictory data. In an attempt to standardize the definition of mesenchymal stem cells, in 2006, the International Society for Cellular Therapy (ISCT) proposed the minimal criteria to define MSCs.¹   See Details

Mesenchymal Stromal Cells or Mesenchymal Stem Cells?

The term ‘mesenchymal stromal cells’ is commonly used to describe a heterogeneous population of cultured cells that are adherent to plastic, have a distinct morphology, and express a specific set of marker proteins. Within this heterogeneous population are cells referred to as ‘mesenchymal stem cells.’   See Details

Kit Components

Positive Markers:

• CD90-APC Mouse IgG_2A; Clone Thy-1A1; 500 μL
• CD73-CFS Mouse IgG_2B; Clone 606112; 500 μL
• CD105-PerCP Mouse IgG₁; Clone 166707; 500 μL

Negative Markers:

• Negative Marker Cocktail (500 μL) that includes:
  • CD45-PE Mouse IgG₁; Clone 2D1
  • CD34-PE Mouse IgG₁; Clone QBEnd10
  • CD11b-PE Mouse IgG_2B; Clone 238446
  • CD79A-PE Mouse IgG₁; Clone 706931
  • HLA-DR-PE Mouse IgG₁; Clone L203

Isotype Controls:

• Mouse IgG_2A-APC Isotype Control; Clone 20102; 500 μL
• Mouse IgG_2B-CFS Isotype Control; Clone 133303; 500 μL
• Mouse IgG₁-PerCP Isotype Control; Clone 11711; 500 μL
• Negative Isotype Cocktail (500 μL) that includes:
  • Mouse IgG₁-PE Isotype Control; Clone 11711
  • Mouse IgG_2B-PE Isotype Control; Clone 133303
• 125 mL of 1X Staining Buffer

Stability and Storage

Store at 2 °C to 8 °C in the dark. Use within 6 months of receipt.

The term 'mesenchymal stem cells' (MSCs) is most commonly used to describe multipotent self-renewing cells that can be differentiated in vitro to generate adipocytes, chondrocytes, and osteoblasts. However, because these biological properties and hierarchical relationships remain to be clearly demonstrated in vivo, the term 'multipotent mesenchymal stromal cells' is often used to distinguish cultured cells from their in vivo precursors. Originally discovered in mouse bone marrow, multipotent mesenchymal stromal cells cultured from a variety of species and tissue types, have been shown to differentiate into progeny of additional lineages including, cardiomyocytes, endothelial cells, hepatocytes, and neural cells. Again, the physiological relevance of these findings remains to be determined.