Human Mesenchymal Stem Cell Verification Flow Kit
Catalog # FMC020 | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Assay Procedure
Refer to the product datasheet for complete product details.
Briefly, mesenchymal stem cells can be analyzed for marker expression using the following procedure:
- Incubate the cells with the provided antibodies
- Wash the cells
- Resuspend in Flow Cytometry Staining Buffer
- Analyze by flow cytometry
Reagents Provided
Kit Components
Reagents supplied in the Human Mesenchymal Stem Cell Marker Verification Multi-Color Flow Cytometry Kit (Catalog # FMC020):
Positive Markers:
- CD90-APC Mouse IgG2A; Clone Thy-1A1; 500 μL
- CD73-CFS Mouse IgG2B; Clone 606112; 500 μL
- CD105-PerCP Mouse IgG1; Clone 166707; 500 μL
Negative Markers:
- Negative Marker Cocktail (500 mL) that includes:
- CD45-PE Mouse IgG1; Clone 2D1
- CD34-PE Mouse IgG1; Clone QBEnd10
- CD11b-PE Mouse IgG2B; Clone 238446
- CD79A-PE Mouse IgG1; Clone 706931
- HLA-DR-PE Mouse IgG1; Clone L203
Isotype Controls:
- Mouse IgG2A-APC Isotype Control; Clone 20102; 500 μL
- Mouse IgG2B-CFS Isotype Control; Clone 133303; 500 μL
- Mouse IgG1-PerCP Isotype Control; Clone 11711; 500 μL
- Negative Isotype Cocktail (500 μL) that includes:
- CD45-PE Mouse IgG1; Clone 2D1
- CD34-PE Mouse IgG1; Clone QBEnd10
- CD11b-PE Mouse IgG2B; Clone 238446
- CD79A-PE Mouse IgG1; Clone 706931
- HLA-DR-PE Mouse IgG1; Clone L203
- 125 mL of 1X Staining Buffer
Other Supplies Required
Reagents
- Fc receptor blocking reagents (optional)
Materials
- Flow Cytometry/FACS™ Tubes (5 mL round-bottom polystyrene tubes)
- Pipette Tips and Pipettes
Equipment
- Clinical centrifuge
- Hemocytometer
- Flow Cytometer
Procedure Overview
- Wash cells with 2 mL of Staining Buffer.

- Perform a cell count.

- Add Fc receptor blocking reagents (optional).
- Transfer 1 x 105 cells in 100 μL into a 5 mL Flow Cytometry tube.

- Add 10 μL of each positive antibody and 10 μL of the Negative Marker Cocktail or add 10 μL of each positive isotype control antibody and 10 μL of the Negative Isotype Control Cocktail.
- Incubate the samples for 30-45 minutes at room temperature in the dark.

- Wash the cells with Staining Buffer.
- Resuspend cell pellets in 200-400 μL of Staining Buffer.

- Analyze the cells for marker expression by flow cytometry.

Citations for Human Mesenchymal Stem Cell Verification Flow Kit (10)
Citations are publications that use Bio-Techne products. Selected citations for Human Mesenchymal Stem Cell Verification Flow Kit include:
-
Species: Human
Sample Types: Whole Cells
Yaylac? et al. (2023-05-17), An enzyme-free technique enables the isolation of a large number of adipose-derived stem cells at the bedside Scientific reports
PMID: 37198228 -
Species: Human
Sample Types: Whole Cells
AJ Janvier et al. (2022-10-31), Multimodal analysis of the differential effects of cyclic strain on collagen isoform composition, fibril architecture and biomechanics of tissue engineered tendon Journal of tissue engineering, 2022-113(0):2041731422113.
PMID: 36339372 -
DM Iacomi et al. (2022-08-10), Generation of an Immortalized Human Adipose-Derived Mesenchymal Stromal Cell Line Suitable for Wound Healing Therapy International Journal of Molecular Sciences, 2022-023(16).
PMID: 36012192 -
Species: Human
Sample Types: Whole Cells
B Sikora et al. (2021-10-07), Paracrine activity of adipose derived stem cells on limbal epithelial stem cells Scientific Reports, 2021-111(1):19956.
PMID: 34620960 -
Species: Human
Sample Types: Whole Cells
M Yoshimatsu et al. (2021-02-11), In vivo regeneration of rat laryngeal cartilage with mesenchymal stem cells derived from human induced pluripotent stem cells via neural crest cells Stem Cell Research, 2021-052(0):102233.
PMID: 33607469 -
Species: Human
Sample Types: Whole Cells
I Pountos et al. (2021-01-10), NSAIDs inhibit bone healing through the downregulation of TGF-&beta3 expression during endochondral ossification Injury, 2021-00(0).
PMID: 33472741 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryS Mount et al. (2019-11-04), Physiologic expansion of human heart-derived cells enhances therapeutic repair of injured myocardium Stem Cell Res Ther, 2019-110(1):316.
PMID: 31685023 -
Species: Human
Sample Types: Whole Cells
B Sikora et al. (2019-10-24), Adipose-derived stem cells undergo differentiation after co-culture with porcine limbal epithelial stem cells Stem Cell Res, 2019-141(0):101609.
PMID: 31706096 -
Species: Human
Sample Types: Whole Cells
JM McKinney et al. (2019-01-29), Therapeutic efficacy of intra-articular delivery of encapsulated human mesenchymal stem cells on early stage osteoarthritis Eur Cell Mater, 2019-037(0):42-59.
PMID: 30693466 -
Species: Human
Sample Types: Whole Cells
A Skubis et al. (2017-11-24), Impact of Antibiotics on the Proliferation and Differentiation of Human Adipose-Derived Mesenchymal Stem Cells Int J Mol Sci, 2017-118(12).
PMID: 29186789
There are no citations that match your criteria.