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StemXVivo Mesenchymal Stem Cell Expansion Media

StemXVivo Mesenchymal Stem Cell Expansion Media
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Assay Procedure

Refer to the product datasheet for complete product details.

Briefly, MSCs are cultured in expansion media using the following protocol:

  • Plate MSCs in expansion media
  • Culture MSCs to 80-90% confluency
  • Passage MSCs using fresh expansion media
 

 

Reagents Provided

Reagents supplied in the StemXVivo® Mesenchymal Stem Cell Expansion Media (Catalog # CCM004):

  • 250 mL of MSC expansion media

 

Other Supplies Required

Reagents

  • Penicillin-Streptomycin (100X)
  • Trypsin-EDTA (10X)
  • Phosphate-Buffered Saline (PBS)

Materials

  • MSCs (e.g., Rat Mesenchymal Stem Cells, Catalog # PSC003)
  • 75 cm2 tissue culture flasks
  • 15 mL centrifuge tubes
  • Serological pipettes
  • Pipettes and pipette tips

Equipment

  • 37 °C and 5% CO2 incubator
  • Centrifuge
  • Hemocytometer
  • Inverted microscope
  • 2 °C to 8 °C refrigerator
  • 37 °C water bath

 

Procedure Overview

This protocol has been tested using bone marrow- and/or adipose tissue-derived MSCs. If using a different tissue source or cell line, the protocol below may need to be modified.

Culturing Mesenchymal Stem Cells

Add 3.5 - 4.0 x 105 MSCs resuspended in 20 mL of the pre-warmed StemXVivo® MSC Expansion Media to a T75 flask.

Culturing Mesenchymal Stem Cells

Replace the medium every 3 days with fresh StemXVivo MSC Expansion Media.

Culturing Mesenchymal Stem Cells

Culture the MSCs to 80-90% confluency.

Subculturing Mesenchymal Stem Cells

Remove and discard the media from the T75 tissue culture flask(s) containing the MSCs.

Wash the cells with PBS.

Subculturing Mesenchymal Stem Cells

Add 1-2 mL of pre-warmed trypsin to the MSCs and incubate the flask at 37 °C.

Subculturing Mesenchymal Stem Cells

Transfer the dissociated MSCs to a 15 mL conical tube.

Centrifuge at 400 x g for 5 minutes.

Subculturing Mesenchymal Stem Cells

Resuspend the cell pellet in 5 mL of StemXVivo® MSC Expansion Media.

Subculturing Mesenchymal Stem Cells

Perform a cell count.

Subculturing Mesenchymal Stem Cells

Add 3.5-4.0 x 105 MSCs resuspended in 20 mL of pre-warmed StemXVivo® MSC Expansion Media to each T75 flask.

Subculturing Mesenchymal Stem Cells

Citations (8)

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Customer Reviews (4)

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  • StemXVivo Mesenchymal Stem Cell Expansion Media
    Name: Anonymous
    Verified Customer | Posted 04/04/2018
  • StemXVivo Mesenchymal Stem Cell Expansion Media
    Name: Anonymous
    Verified Customer | Posted 03/23/2018
    I am working on bone marrow stem cells.StemXVivo MSC expansion medium help to retain the population doubling time from P-4 to P-8 30 hrs to 34 hrs only, where when I culture these MSC in normal alpha MEM after some time cells do not divide/proliferate.
  • StemXVivo Mesenchymal Stem Cell Expansion Media
    Name: Anonymous
    Verified Customer | Posted 12/06/2016
  • StemXVivo Mesenchymal Stem Cell Expansion Media
    Name: Anonymous
    Verified Customer | Posted 12/06/2016
    I used this media for my umbilical cord-derived MSC, after speaking with a representative, who gave me a sample. I tried the product because I saw my cells were growing really small in another brand product. After switching, the rate of growth of my cells dramatically increased, and I could not be happier than that!

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FAQs

  • Does StemXVivo® Mesenchymal Stem Cell Expansion Media need addition of serum before use?

    The StemXVivo® Mesenchymal Stem Cell Expansion Media does not need addition of serum. Fetal bovine serum is included in the product, which is a complete medium and ready to use. The media may be supplemented with cytokine or growth factors for your desired cell culture application.

  • Does StemXVivo® Mesenchymal Stem Cell Expansion Media (Catalog # CCM004) contain Phenol Red?

    Yes,StemXVivo® Mesenchymal Stem Cell Expansion Media contains Phenol Red.

  • How long can Mesenchymal Stem Cells (MSCs) be cultured in StemXVivo® Mesenchymal Stem Cell Expansion Media (Catalog # CCM004)?

    MSCs can be grown to 80-90% confluency and subsequently subcultured using the protocol provided in the product datasheet. Researchers should establish the number of passages that is acceptable for their work. MSCs are sensitive to passsages and, if subcultured too many times, may start losing their MSC characteristics. Our MSC Functional Identification Kits can be used for validation of MSC mulitpotency.

  • Can Mesenchymal Stem Cells (MSCs) grown in StemXVivo® MSC Expansion Media (Catalog # CCM004) be cryopreserved?

    MSCs grown in StemXVivo® MSC Expansion Media can be cryopreserved using appropriate cryopreservation media, such as StemXVivo® Serum-Free MSC Freezing Media (Catalog # CCM016) or equivalent.

Product Datasheets

Certificate of Analysis

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