StemXVivo Mesenchymal Stem Cell Expansion Media
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$35 to United States
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Assay Procedure
Refer to the product datasheet for complete product details.
Briefly, MSCs are cultured in expansion media using the following protocol:
- Plate MSCs in expansion media
- Culture MSCs to 80-90% confluency
- Passage MSCs using fresh expansion media
Reagents supplied in the StemXVivo® Mesenchymal Stem Cell Expansion Media (Catalog # CCM004):
- 250 mL of MSC expansion media
Reagents
- Penicillin-Streptomycin (100X)
- Trypsin-EDTA (10X)
- Phosphate-Buffered Saline (PBS)
Materials
- MSCs (e.g., Rat Mesenchymal Stem Cells, Catalog # PSC003)
- 75 cm2 tissue culture flasks
- 15 mL centrifuge tubes
- Serological pipettes
- Pipettes and pipette tips
Equipment
- 37 °C and 5% CO2 incubator
- Centrifuge
- Hemocytometer
- Inverted microscope
- 2 °C to 8 °C refrigerator
- 37 °C water bath
This protocol has been tested using bone marrow- and/or adipose tissue-derived MSCs. If using a different tissue source or cell line, the protocol below may need to be modified.
Culturing Mesenchymal Stem Cells
Add 3.5 - 4.0 x 105 MSCs resuspended in 20 mL of the pre-warmed StemXVivo® MSC Expansion Media to a T75 flask.

Replace the medium every 3 days with fresh StemXVivo MSC Expansion Media.

Culture the MSCs to 80-90% confluency.
Subculturing Mesenchymal Stem Cells
Remove and discard the media from the T75 tissue culture flask(s) containing the MSCs.
Wash the cells with PBS.

Add 1-2 mL of pre-warmed trypsin to the MSCs and incubate the flask at 37 °C.

Transfer the dissociated MSCs to a 15 mL conical tube.
Centrifuge at 400 x g for 5 minutes.

Resuspend the cell pellet in 5 mL of StemXVivo® MSC Expansion Media.

Perform a cell count.

Add 3.5-4.0 x 105 MSCs resuspended in 20 mL of pre-warmed StemXVivo® MSC Expansion Media to each T75 flask.

Citations (8)
Citations are publications that use Bio-techne products. Selected citations for StemXVivo Mesenchymal Stem Cell Expansion Media include:
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Species: Human
Sample Types: Whole Cells
Applications: BioassayKK Brodaczews et al. (2019), Metastatic renal cell carcinoma cells growing in 3D on poly?D?lysine or laminin present a stem?like phenotype and drug resistance Oncol. Rep., 42(5):1878-1892.
PMID: 31545459 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayC Galeano et al. (2018), The Route by Which Intranasally Delivered Stem Cells Enter the Central Nervous System Cell Transplant, 0(0):9636897187545.
PMID: 29756518 -
Species: Mouse
Sample Types: Whole Cells
M Fukuda et al. (2018), SIRT7 has a critical role in bone formation by regulating lysine acylation of SP7/Osterix Nat Commun, 9(1):2833.
PMID: 30026585 -
Species: Mouse
Sample Types: Whole Cells
Applications: BioassayR Fajka-Boja et al. (2018), Increased insulin-like growth factor 1 production by polyploid adipose stem cells promotes growth of breast cancer cells BMC Cancer, 18(1):872.
PMID: 30185144 -
Species: Rat
Sample Types: Whole Cells
Sawitza I et al. (2015), Bile acids induce hepatic differentiation of mesenchymal stem cells. Sci Rep, 5(0):13320.
PMID: 26304833 -
Species: Mouse
Sample Types: Whole Cells
Applications: Cell CultureTibboel et al. (2014), Intravenous and intratracheal mesenchymal stromal cell injection in a mouse model of pulmonary emphysema. COPD, 11(3):310-8.
PMID: 24295402 -
Species: Mouse
Sample Types: Whole Cells
Applications: Cell CultureLai D et al. (2014), Skin-derived mesenchymal stem cells help restore function to ovaries in a premature ovarian failure mouse model. PLoS ONE, 9(5):e98749.
PMID: 24879098 -
Species: Human
Sample Types: Whole Cells
Cui et al. (2012), Dynamic microRNA profiles of hepatic differentiated human umbilical cord lining-derived mesenchymal stem cells. PLoS ONE, 7(9):e44737.
PMID: 22984549
There are no citations that match your criteria.
Customer Reviews (4)
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StemXVivo Mesenchymal Stem Cell Expansion Media
Verified Customer | Posted 04/04/2018 -
StemXVivo Mesenchymal Stem Cell Expansion Media
Verified Customer | Posted 03/23/2018I am working on bone marrow stem cells.StemXVivo MSC expansion medium help to retain the population doubling time from P-4 to P-8 30 hrs to 34 hrs only, where when I culture these MSC in normal alpha MEM after some time cells do not divide/proliferate. -
StemXVivo Mesenchymal Stem Cell Expansion Media
Verified Customer | Posted 12/06/2016 -
StemXVivo Mesenchymal Stem Cell Expansion Media
Verified Customer | Posted 12/06/2016I used this media for my umbilical cord-derived MSC, after speaking with a representative, who gave me a sample. I tried the product because I saw my cells were growing really small in another brand product. After switching, the rate of growth of my cells dramatically increased, and I could not be happier than that!
There are no reviews that match your criteria.
FAQs
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Does StemXVivo® Mesenchymal Stem Cell Expansion Media need addition of serum before use?
The StemXVivo® Mesenchymal Stem Cell Expansion Media does not need addition of serum. Fetal bovine serum is included in the product, which is a complete medium and ready to use. The media may be supplemented with cytokine or growth factors for your desired cell culture application.
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Does StemXVivo® Mesenchymal Stem Cell Expansion Media (Catalog # CCM004) contain Phenol Red?
Yes,StemXVivo® Mesenchymal Stem Cell Expansion Media contains Phenol Red.
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How long can Mesenchymal Stem Cells (MSCs) be cultured in StemXVivo® Mesenchymal Stem Cell Expansion Media (Catalog # CCM004)?
MSCs can be grown to 80-90% confluency and subsequently subcultured using the protocol provided in the product datasheet. Researchers should establish the number of passages that is acceptable for their work. MSCs are sensitive to passsages and, if subcultured too many times, may start losing their MSC characteristics. Our MSC Functional Identification Kits can be used for validation of MSC mulitpotency.
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Can Mesenchymal Stem Cells (MSCs) grown in StemXVivo® MSC Expansion Media (Catalog # CCM004) be cryopreserved?
MSCs grown in StemXVivo® MSC Expansion Media can be cryopreserved using appropriate cryopreservation media, such as StemXVivo® Serum-Free MSC Freezing Media (Catalog # CCM016) or equivalent.