Human Mesenchymal Stem Cell Multi-Color Flow Kit
R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Assay Procedure
Refer to the product datasheet for complete product details.
Reagents Provided
Reagents Supplied in the Human Mesenchymal Stromal Cell Multi-Color Flow Cytometry Kit (Catalog # FMC002):
- Flow Cytometry Staining Buffer (100 mL)
Positive Markers
- Mouse IgG1 Anti-Human CD105-PerCP (Clone 166707)
- Mouse IgG1 Anti-Human CD146-CFS (Clone 128018)
- Mouse IgG2A Anti-Human CD90-APC (Clone Thy-1A1)
Negative Markers
- Mouse IgG1 Anti-Human CD45-PE (Clone 2D1)
Other Supplies Required
Reagents
- Fc Receptor Blocking Reagents
Materials
- Flow Cytometry/FACS™ Tubes (5 mL round-bottom polystyrene tubes)
- Pipette Tips and Pipettes
Equipment
- Centrifuge
- Vortex
Procedure Overview
This protocol has been tested using bone marrow- and/or adipose tissue-derived MSCs. If using a different tissue source or cell line, the protocol below may need to be optimized.






- Wash the cells with Flow Cytometry Staining Buffer.
Perform a cell count. - Add Fc receptor blocking reagents. If using excess pre-immune IgG to block Fc receptor, the excess IgG does not need to be washed from the cells following the incubation period.
- Transfer approximately 100 µL of the Fc receptor-blocked cells (about 1 x 106 cells) into a 5 mL flow cytometry tube.
- Add 10 µL of each fluorochrome-conjugated antibody or each corresponding isotype control antibody.
- Incubate the mixture for 30-45 minutes at room temperature in the dark.
- Centrifuge the samples at 300 x g for 5 minutes.
- Wash the cells with 2 mL Flow Cytometry Staining Buffer.
- Resuspend the cell pellet in 200-400 µL of Flow Cytometry Staining Buffer.
- Analyze expression of MSC markers simultaneously by flow cytometry.
Citations for Human Mesenchymal Stem Cell Multi-Color Flow Kit (4)
Citations are publications that use Bio-Techne products. Selected citations for Human Mesenchymal Stem Cell Multi-Color Flow Kit include:
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Species: Human
Sample Types: Whole Cells
M Horiguchi et al. (2021), The Transplantation Resistance of Type II Diabetes Mellitus Adipose-Derived Stem Cells Is Due to G6PC and IGF1 Genes Related to the FoxO Signaling Pathway International Journal of Molecular Sciences, 22(12).
PMID: 34205470 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryTS Winston et al. (2019), Serum-Free Manufacturing of Mesenchymal Stem Cell Tissue Rings Using Human-Induced Pluripotent Stem Cells Stem Cells Int, 2019(0):5654324.
PMID: 30766604 -
Species: Human
Sample Types: Whole Cells
S Izumi et al. (2019), Control of glucose metabolism is important in tenogenic differentiation of progenitors derived from human injured tendons PLoS ONE, 14(3):e0213912.
PMID: 30883580 -
Species: Human
Sample Types: Whole Cells
M Valverde et al. (2018), Hydrogen Peroxide-Induced DNA Damage and Repair through the Differentiation of Human Adipose-Derived Mesenchymal Stem Cells Stem Cells Int, 2018(0):1615497.
PMID: 30405718
There are no citations that match your criteria.
Customer Reviews for Human Mesenchymal Stem Cell Multi-Color Flow Kit (1)
FAQs for Human Mesenchymal Stem Cell Multi-Color Flow Kit
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Will the Human Mesenchymal Stem Cell Multi-Color Flow Kit (catalog # FMC002) or Mouse Mesenchymal Stem Cell Multi-Color Flow Kit (catalog # FMC003) work for identificaiton of Rat Menchymal stem cells?
The Human Mesenchymal Stem Cell Multi-Color Flow Kit (catalog # FMC002) or Mouse Mesenchymal Stem Cell Multi-Color Flow Kit (catalog # FMC003) will not work for identificaiton of Rat Mesenchymal stem cells.