Will the Human Mesenchymal Stem Cell Multi-Color Flow Kit (catalog # FMC002) or Mouse Mesenchymal Stem Cell Multi-Color Flow Kit (catalog # FMC003) work for identificaiton of Rat Menchymal stem cells?

Name: Human Mesenchymal Stem Cell Multi-Color Flow Kit
Brand: R&D Systems
SKU: FMC002
Rating: 4 (1 reviews)

The Human Mesenchymal Stem Cell Multi-Color Flow Kit (catalog # FMC002) or Mouse Mesenchymal Stem Cell Multi-Color Flow Kit (catalog # FMC003) will not work for identificaiton of Rat Mesenchymal stem cells.

Human Mesenchymal Stem Cell Multi-Color Flow Kit

R&D Systems, Inc. a Bio-Techne Brand

Catalog # FMC002

Citations (4)

(1)

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Setting the standard in quality research reagents

Summary for Human Mesenchymal Stem Cell Multi-Color Flow Kit

Kit Summary

For verification of mesenchymal stem/stromal cell identity by flow cytometry.

Key Benefits

Verifies MSC identity in less than 2 hours
Conjugated antibodies allow for single step staining
Defines the starting cell population
Multiple markers reduce experimental variation

Why is it important to verify MSC identity?

Human mesenchymal stem/stromal cells (MSCs) can be isolated by a variety of techniques, grown and expanded using numerous reagents, and differentiated with a range of media, proteins, and small molecules.
See Details

Variations in techniques as well as differences in the MSC starting population may account for experimental variability and some of the contradictory data in the literature. By clearly defining the starting cell populations, researchers can decrease experimental variability and ultimately decrease apparent discrepancies in the literature that stem from the use of phenotypically different cell populations. R&D System offers the Human Mesenchymal Stem Cell Multi-Color Flow Cytometry Kit which is used to define human MSCs based on established markers. The Human MSC Multi-color Flow Cytometry Kit includes four fluorochrome-conjugated antibodies for the detection of MSC markers by flow cytometry.

The Human Mesenchymal Stromal Cell Multi-Color Flow Cytometry Kit:

Efficiently verifies MSC identity using flow cytometry.
Defines the starting cell population to reduce experimental variability.
Includes conjugated-antibodies to reduce hands-on time.
Includes multiple MSC markers to increase confidence in MSC identity.

Mesenchymal Stromal Cells or Mesenchymal Stem Cells?

The term ‘mesenchymal stromal cells’ is commonly used to describe a heterogeneous population of cultured cells that are adherent to plastic, have a distinct morphology, and express a specific set of marker proteins. Within this heterogeneous population are cells referred to as ‘mesenchymal stem cells.’ See Details

Mesenchymal stem cells are multipotent, self-renewing cells that have the ability to differentiate into adipocytes, chondrocytes, and osteoblasts when cultured in vitro.

2006 Proposed Change to MSC Nomenclature

Although mesenchymal stromal cells were once referred to as ‘mesenchymal stem cells,’ a change to ‘mesenchymal stromal cells’ was proposed by the International Society for Cellular Therapy in 2006.¹

The change in nomenclature originates from two important factors:

Methods used to isolate mesenchymal stem cells yield a heterogeneous population of cells with only a fraction of these cells demonstrating multipotency.
The absence of direct evidence that mesenchymal stem cells can self-renew and differentiate in vivo.

Use of Mesenchymal Stem and Stromal Cell Terminology

Data supporting MSC self-renewal and multipotency have been obtained using in vitro conditions, which does not adequately reflect the in vivo environment. The lack of in vivo data has led some researchers to question the validity of the term ‘mesenchymal stem cell’ providing further support for the use of ‘mesenchymal stromal cells’ to describe MSCs.² While ‘mesenchymal stromal cells’ may be the more scientifically accurate term for MSCs, the two terms are often used interchangeably in the literature. R&D Systems recognizes the use of both mesenchymal stem cells and mesenchymal stromal cells and uses ‘MSC’ to indicate mesenchymal stem/stromal cells to account for both designations.

Definitions of Mesenchymal Stromal Cells and Mesenchymal Stem Cells

Mesenchymal Stromal Cells – A heterogeneous population of cultured cells with similar characteristics such as the ability to adhere to plastic and the expression of specific marker proteins.
Mesenchymal Stem Cells – A subpopulation of mesenchymal stromal cells that have the capacity to self-renew and differentiate into mesodermal lineages when cultured in vitro. The capacity to self-renew and differentiate in vivo has yet to be clearly demonstrated for mesenchymal stem cells.

References

Dominici, M. et al. (2006) Cytotherapy 8:315.
Keating, A. (2012) Cell Stem Cell 10:709.

Kit Components

The Human Mesenchymal Stromal Cell Multi-Color Flow Cytometry Kit contains four conjugated monoclonal antibodies and corresponding isotype controls that can be used for single-step staining of human mesenchymal stem/stromal cells. This kit includes sufficient reagents for 25 assays. See Details

Flow Cytometry Staining Buffer (100 mL)

Positive Markers

Mouse IgG₁ Anti-Human CD105-PerCP (Clone 166707)
Mouse IgG₁ Anti-Human CD146-CFS (Clone 128018)
Mouse IgG_2A Anti-Human CD90-APC (Clone Thy-1A1)

Negative Markers

Mouse IgG₁ Anti-Human CD45-PE (Clone 2D1)

Stability and Storage

Store at 2 °C to 8 °C in the dark. Use within 6 months of receipt.

Precautions

The Staining Buffer contains 0.1% sodium azide. Sodium azide may react with lead and copper plumbing to form explosive metallic azides. Flush with large volumes of water during disposal.

The term 'mesenchymal stem cells' (MSCs) is most commonly used to describe multipotent self-renewing cells that can be differentiated in vitro to generate adipocytes, chondrocytes, and osteoblasts. However, because these biological properties and hierarchical relationships remain to be clearly demonstrated in vivo, the term 'multipotent mesenchymal stromal cells' is often used to distinguish cultured cells from their in vivo precursors. Originally discovered in mouse bone marrow, multipotent mesenchymal stromal cells cultured from a variety of species and tissue types, have been shown to differentiate into progeny of additional lineages including, cardiomyocytes, endothelial cells, hepatocytes, and neural cells. Again, the physiological relevance of these findings remains to be determined.

Product Specifications for Human Mesenchymal Stem Cell Multi-Color Flow Kit

Species

Human

Scientific Data Examples for Human Mesenchymal Stem Cell Multi-Color Flow Kit

	Verification of Mesenchymal Stem/Stromal Cell Identity by Analysis of Mesenchymal Stem/Stromal Cell Marker Expression. Undifferentiated (A) and osteocyte-differentiated (B) human mesenchymal stem cells were stained with the indicated antibodies (filled histograms) or the corresponding isotype control (open histograms), using the Human Mesenchymal Stromal Cell Multi-Color Flow Cytometry Kit (Catalog # FMC002). Multi-color flow cytometry simultaneously detected cells that were positive for MSC markers (CD105/Endoglin, CD146/MCAM, and CD90/Thy1). Following 21 days of osteocyte-differentiation, the cells showed a characteristic reduction in CD105/Endoglin, CD146/MCAM, and CD90/Thy1 staining. CD45 is a negative control for both cell types.

Preparation & Storage

Shipping Conditions	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Assay Procedure

Refer to the product datasheet for complete product details.

Briefly, the starting population of cells is characterized for MSC markers using the following procedure:

Incubate the cells with the provided antibodies
Wash the cells
Resuspend in Flow Cytometry Staining Buffer
Analyze by flow cytometry

View Graphic Procedure

Reagents Provided

Reagents Supplied in the Human Mesenchymal Stromal Cell Multi-Color Flow Cytometry Kit (Catalog # FMC002):

Flow Cytometry Staining Buffer (100 mL)

Positive Markers

Mouse IgG₁ Anti-Human CD105-PerCP (Clone 166707)
Mouse IgG₁ Anti-Human CD146-CFS (Clone 128018)
Mouse IgG_2A Anti-Human CD90-APC (Clone Thy-1A1)

Negative Markers

Mouse IgG₁ Anti-Human CD45-PE (Clone 2D1)

Other Supplies Required

Reagents

Fc Receptor Blocking Reagents

Materials

Flow Cytometry/FACS™ Tubes (5 mL round-bottom polystyrene tubes)
Pipette Tips and Pipettes

Equipment

Centrifuge
Vortex

Procedure Overview

This protocol has been tested using bone marrow- and/or adipose tissue-derived MSCs. If using a different tissue source or cell line, the protocol below may need to be optimized.

Wash the cells with Flow Cytometry Staining Buffer.
Perform a cell count.
Add Fc receptor blocking reagents. If using excess pre-immune IgG to block Fc receptor, the excess IgG does not need to be washed from the cells following the incubation period.
Transfer approximately 100 µL of the Fc receptor-blocked cells (about 1 x 10⁶ cells) into a 5 mL flow cytometry tube.
Add 10 µL of each fluorochrome-conjugated antibody or each corresponding isotype control antibody.
Incubate the mixture for 30-45 minutes at room temperature in the dark.
Centrifuge the samples at 300 x g for 5 minutes.
Wash the cells with 2 mL Flow Cytometry Staining Buffer.
Resuspend the cell pellet in 200-400 µL of Flow Cytometry Staining Buffer.
Analyze expression of MSC markers simultaneously by flow cytometry.

Citations for Human Mesenchymal Stem Cell Multi-Color Flow Kit (4)

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Citations are publications that use Bio-Techne products. Selected citations for Human Mesenchymal Stem Cell Multi-Color Flow Kit include:

Species: Human
Sample Types: Whole Cells

M Horiguchi et al. (2021), The Transplantation Resistance of Type II Diabetes Mellitus Adipose-Derived Stem Cells Is Due to G6PC and IGF1 Genes Related to the FoxO Signaling Pathway International Journal of Molecular Sciences, 22(12).
PMID: 34205470
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

TS Winston et al. (2019), Serum-Free Manufacturing of Mesenchymal Stem Cell Tissue Rings Using Human-Induced Pluripotent Stem Cells Stem Cells Int, 2019(0):5654324.
PMID: 30766604
Species: Human
Sample Types: Whole Cells

S Izumi et al. (2019), Control of glucose metabolism is important in tenogenic differentiation of progenitors derived from human injured tendons PLoS ONE, 14(3):e0213912.
PMID: 30883580
Species: Human
Sample Types: Whole Cells

M Valverde et al. (2018), Hydrogen Peroxide-Induced DNA Damage and Repair through the Differentiation of Human Adipose-Derived Mesenchymal Stem Cells Stem Cells Int, 2018(0):1615497.
PMID: 30405718

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FAQs for Human Mesenchymal Stem Cell Multi-Color Flow Kit

Will the Human Mesenchymal Stem Cell Multi-Color Flow Kit (catalog # FMC002) or Mouse Mesenchymal Stem Cell Multi-Color Flow Kit (catalog # FMC003) work for identificaiton of Rat Menchymal stem cells?

The Human Mesenchymal Stem Cell Multi-Color Flow Kit (catalog # FMC002) or Mouse Mesenchymal Stem Cell Multi-Color Flow Kit (catalog # FMC003) will not work for identificaiton of Rat Mesenchymal stem cells.

Human Mesenchymal Stem Cell Multi-Color Flow Kit

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Summary for Human Mesenchymal Stem Cell Multi-Color Flow Kit

Kit Summary

Key Benefits

Why is it important to verify MSC identity?

Mesenchymal Stromal Cells or Mesenchymal Stem Cells?

2006 Proposed Change to MSC Nomenclature

Use of Mesenchymal Stem and Stromal Cell Terminology

Definitions of Mesenchymal Stromal Cells and Mesenchymal Stem Cells

References

Kit Components

Positive Markers

Negative Markers

Stability and Storage

Precautions

Product Specifications for Human Mesenchymal Stem Cell Multi-Color Flow Kit

Scientific Data Examples for Human Mesenchymal Stem Cell Multi-Color Flow Kit

Preparation & Storage

Assay Procedure

Reagents Provided

Reagents Supplied in the Human Mesenchymal Stromal Cell Multi-Color Flow Cytometry Kit (Catalog # FMC002):

Positive Markers

Negative Markers

Other Supplies Required

Reagents

Materials

Equipment

Procedure Overview

Citations for Human Mesenchymal Stem Cell Multi-Color Flow Kit (4)

Customer Reviews for Human Mesenchymal Stem Cell Multi-Color Flow Kit (1)

Customer Images

Human Mesenchymal Stem Cell Multi-Color Flow Kit

FAQs for Human Mesenchymal Stem Cell Multi-Color Flow Kit

Product Documents for Human Mesenchymal Stem Cell Multi-Color Flow Kit

Product Datasheets

COA

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SDS

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