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Rat TRACP/PAP/ACP5 ELISA Kit (Colorimetric), Novus Biologicals

Bio-Techne includes Novus Biologicals | Catalog # NBP2-76464

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NBP2-76464

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.38 ng/mL

Assay Range

0.63 - 40 ng/mL

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This kit recognizes Rat ACP5 in samples. No significant cross-reactivity or interference between Rat ACP5 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 4.5%

Inter-Assay Precision (Precision between assays) CV% < 4.71%

Recovery for Rat TRACP/PAP/ACP5 ELISA Kit (Colorimetric)

Recovery

84-109%

Linearity

Rat TRACP/PAP/ACP5 ELISA Kit (Colorimetric)

Scientific Data Images for Rat TRACP/PAP/ACP5 ELISA Kit (Colorimetric)

Rat TRACP/PAP/ACP5 ELISA Kit (Colorimetric)

ELISA: Rat TRACP/PAP/ACP5 ELISA Kit (Colorimetric) [NBP2-76464] -

ELISA: Rat TRACP/PAP/ACP5 ELISA Kit (Colorimetric) [NBP2-76464] - Standard Curve Reference

Kit Contents for Rat TRACP/PAP/ACP5 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Certificate of Analysis
  • Concentrated Biotinylated Detection Ab (100×)
  • Concentrated HRP Conjugate (100×)
  • Concentrated Wash Buffer (25×)
  • HRP Conjugate Diluent
  • Micro ELISA Plate(Dismountable)
  • Plate Sealer
  • Product Description
  • Reference Standard
  • Reference Standard & Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: TRACP/PAP/ACP5

TRACP is an iron-containing, 35 kDa glycoprotein that hydrolyses phosphomonoesters. It is produced as a monomer by osteoclasts and is cleaved by cathepsins to create a disulfide-linked dimer that acts on bone matrix phosphoserine-containing proteins in lysosomal vesicles. Following phosphate cleavage, TRACP is released from the cell where it serves as a marker of bone resorption.

Long Name

Tartrate Resistant Acid Phosphatase 5

Alternate Names

ACP5, TRACP-5b, TTRRAP

Gene Symbol

ACP5

Additional TRACP/PAP/ACP5 Products

Product Documents for Rat TRACP/PAP/ACP5 ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat TRACP/PAP/ACP5 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

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