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Total Nitric Oxide and Nitrate/Nitrite Parameter Assay Kit

Multi-species Total Nitric Oxide and Nitrate/Nitrite ELISA Standard Curve
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Multi-species Total Nitric Oxide and Nitrate/Nitrite ELISA Linearity
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KGE001
SKGE001
PKGE001

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Key Product Details

Assay Length: < 1 hour
Sample Type & Volume Required Per Well: Cell Culture Supernates (10 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL), Citrate Plasma (25 uL), Urine (10 uL)
Sensitivity: 0.78 umol/L
Assay Range: 3.12 - 200 umol/L (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine)
Total Nitric Oxide and Nitrate/Nitrite Parameter Assay Kit Summary: The Parameter Total Nitric Oxide kit has two assay options. Endogenous nitrite is measured in the first option. In the second option, nitrate is converted to nitrite using nitrate reductase and total nitrite is measured. To obtain the nitrate concentration, endogenous nitrite is subtracted from the total nitrite value.

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Customer Reviews (5)

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  • Total Nitric Oxide and Nitrate/Nitrite Parameter Assay Kit
    Name: Anonymous
    Sample Tested: Cell culture supernatant
    Verified Customer | Posted 10/19/2020
    Total Nitric Oxide and Nitrate/Nitrite Parameter Assay Kit Serum KGE001
  • Total Nitric Oxide and Nitrate/Nitrite Parameter Assay Kit
    Name: Anonymous
    Sample Tested: THP-1 human acute monocytic leukemia cell line
    Verified Customer | Posted 03/12/2019
    Total Nitric Oxide and Nitrate/Nitrite Parameter Assay Kit Serum KGE001
    Bio-Techne Response
    Thank you for reviewing our product. We are sorry to hear that this antibody did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.
  • Total Nitric Oxide and Nitrate/Nitrite Parameter Assay Kit
    Name: Anonymous
    Sample Tested: Cell culture supernatant
    Verified Customer | Posted 02/26/2019
    Total Nitric Oxide and Nitrate/Nitrite Parameter Assay Kit Serum KGE001
  • Total Nitric Oxide and Nitrate/Nitrite Parameter Assay Kit
    Name: Anonymous
    Sample Tested: Cell Culture Media
    Verified Customer | Posted 04/02/2018
  • Total Nitric Oxide and Nitrate/Nitrite Parameter Assay Kit
    Name: Anonymous
    Sample Tested: Human serum
    Verified Customer | Posted 08/23/2016
    I did spike in study for both nitrite and nitrate assay. The recovery of Nitrite is very good. However the nitrate is very poor.
    Total Nitric Oxide and Nitrate/Nitrite Parameter Assay Kit Serum KGE001

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