Testosterone Parameter Assay Kit Best Seller
R&D Systems, part of Bio-Techne | Catalog # KGE010
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 µL), Serum (20 µL), EDTA Plasma (20 µL), Heparin Plasma (20 µL)
Sensitivity
0.041 ng/mL
Assay Range
0.041-10 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Testosterone Parameter Assay Kit
The Parameter Testosterone kit is a 4.5 hour competitive enzyme immunoassay designed to measure Testosterone in cell culture supernates, serum, and plasma.
Product Specifications
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
Biotin
Reactivity
Multi-Species
Specificity
Measures Testosterone levels in various samples
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules. Cross-species reactivity not tested.
Interference
Interference observed with 1 or more available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (ng/mL) | 1.25 | 3.50 | 5.80 | 1.26 | 3.67 | 6.03 |
| Standard Deviation | 0.05 | 0.11 | 0.17 | 0.07 | 0.23 | 0.41 |
| CV% | 4.0 | 3.1 | 2.9 | 5.6 | 6.3 | 6.8 |
Recovery for Testosterone Parameter Assay Kit
The recovery of Testosterone spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 103 | 95-117 |
| EDTA Plasma (n=4) | 94 | 85-107 |
| Heparin Plasma (n=4) | 92 | 84-101 |
| Serum (n=4) | 93 | 86-104 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Testosterone were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Testosterone Parameter Assay Kit
Multi-species Testosterone ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Testosterone
Alternate Names
17b-Hydroxyandrost-4-ene-3-one, 17b-Testosterone
Additional Testosterone Products
Product Documents for Testosterone Parameter Assay Kit
Product Specific Notices for Testosterone Parameter Assay Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.