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SARS-CoV-2 Variant Inhibitor Screening Kit

COVID-19
Omicron Variant Now Availble, Catalog # VANC00B!
(2)
Data showing blocking ability of antibodies from vaccinated individuals to disrupt the Spike protein to ACE-2 interaction in ELISA
Discontinued Product
VANC00 has been discontinued and is replaced by VANC00B.

Key Product Details

Assay Type: ELISA Type Binding Assay
Sample Types: Cell Lysates
SARS-CoV-2 Variant Inhibitor Screening Kit Features:

See the Data: Read our white paper on testing individual serum samples using this kit. Download White Paper

 

Assay Workflow: 5 Simple Steps

 

Product Features

  • Quickly and efficiently evaluates the effectiveness of vaccines against SARS-CoV-2 variants
  • Validated step by step assay protocol
  • No special equipment needed
  • Can be used for high throughput screening SARS-CoV-2 inhibitors

Background: Severe acute respiratory syndrome Coronavirus-2

Severe acute respiratory syndrome Coronavirus-2 (SARS-CoV-2), the virus that causes COVID-19, has posed a serious threat to global human health. With the emergence of variants, especially the Variants of Concern (VOCs), a quick and effective way to assess how effective the current vaccines are against the major variants is needed. The COVID-19 Neutralization Kit is an ELISA type of binding assay measuring the ability of the sera of vaccinated donors to block the binding between RBDs of COVID-19 variants and human ACE-2.

 

Complete Your Research

Citations for SARS-CoV-2 Variant Inhibitor Screening Kit

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FAQs

  • What can cause high background?

    Problem of high background may be due to insufficient washing or extended incubation time with detection reagents, among other factors.

  • What are possible reasons for low assay signal?

    Low assay signal may be due to non-optimal preparation of Recombinant SARS2 Spike RBD proteins or Biotinylated Human ACE-2. Be sure to review the product insert and follow reconstitution and dilution instructions for each lyophilized reagent. Low signal may also be caused by insufficient incubation time for color reaction. Color development for the Covid-19 Neutralization assay requires 15 to 25 minutes.

  • What could cause high variability between sample duplicates?

    The two main reasons for high variability in this assay are non-optimal pipetting technique and samples not well mixed. Ensure a consistent and accurate pipetting method. Mix sample thoroughly and dispense accurately. Change pipette tips between samples. Ensure that your pipettes are calibrated.

  • Is it okay to modify the plate layout in the kit product datasheet?

    Yes, a plate layout is provided in the kit insert as an example, and investigators can modify the plate layout to suit specific needs. Spike RBD WT or variant proteins are supplied at higher amounts to provide flexibility for investigators to use each protein of interest as desired.

  • Why are Sample Dilution Plates provided in the kit?

    The Sample Dilution Plates have reduced protein binding to minimize loss of sample during the dilution and pre-incubation steps, and the plates are provided in the kit for convenience of use. Diluted samples prepared in a dilution plate can be transferred to a 96-well Assay Plate in an efficient manner minimizing chance for assay drift.

  • The SARS-CoV-2 variants that I am interested in are not listed in the kit insert. Is there an option to obtain a kit that contains other variants?

    Yes; to discuss other available options, please contact our Technical Support by email techsupport@bio-techne.com or by phone Toll Free USA, Canada: 1-800-343-7475Phone (International): 1-800-343-7475.

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.