SARS-CoV-2 Variant Inhibitor Screening KitNew

Assay Workflow: 5 Simple Steps

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Product Features

• Quickly and efficiently evaluates the effectiveness of vaccines against SARS-CoV-2 variants
• Validated step by step assay protocol
• No special equipment needed
• Can be used for high throughput screening SARS-CoV-2 inhibitors

Kit Contents

• Capture Antibody
• SARS-Cov-2 Spike RBD Wild Type
• SARS-CoV-2 Spike RBD Variants (Alpha, beta, Gamma and Delta)
• Biotinylated Human ACE-2
• Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Preparation and Storage

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Severe acute respiratory syndrome Coronavirus-2

Severe acute respiratory syndrome Coronavirus-2 (SARS-CoV-2), the virus that causes COVID-19, has posed a serious threat to global human health. With the emergence of variants, especially the Variants of Concern (VOCs), a quick and effective way to assess how effective the current vaccines are against the major variants is needed. The COVID-19 Neutralization Kit is an ELISA type of binding assay measuring the ability of the sera of vaccinated donors to block the binding between RBDs of COVID-19 variants and human ACE-2.

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)