Human SAA ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # KA0518

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Cell Culture Supernatant, Plasma, Serum (100 uL)

Sensitivity

1.1 ng/mL

Assay Range

1.1 to 80 ng/mL

View all SAA ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Description

Quality control test: Standard curve. SAA (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of human SAA.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 4.00%

Inter-Assay Precision (Precision between assays) CV% < 7.90%

Recovery for Human SAA ELISA Kit (Colorimetric)

Recovery

Serum: 104.00%, Plasma: 104.80%, Cell Culture Supernatant: 98.20%

Scientific Data Images for Human SAA ELISA Kit (Colorimetric)

ELISA: Human SAA ELISA Kit (Colorimetric) [KA0518]

ELISA: Human SAA ELISA Kit (Colorimetric) [KA0518]

ELISA: SAA ELISA Kit [HRP] [KA0518] - Standard curve

Kit Contents for Human SAA ELISA Kit (Colorimetric)

  • Antibody coated microwells
  • CALIBRATOR DILUENT I
  • CALIBRATOR DILUENT II
  • SAA CONJUGATE
  • SAA STANDARD
  • Stop Solution
  • SUBSTRATE A
  • SUBSTRATE B
  • WASH BUFFER (20X)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: SAA

The serum amyloid A (SAA) family comprises a number of differentially expressed apolipoproteins, acute-phase SAA1 and SAA2, the former being the major component in plasma, and constitutive SAAs (C-SAAs). Although the liver is the primary site of synthesis of both SAA types extrahepatic production has been reported. The in vivo concentrations increase by as much as 1000-fold during inflammation. Several studies have stressed its importance in the diagnosis and monitoring of various diseases. Pathological SAA values are often detected in association with normal CRP concentrations; SAA rises earlier and more sharply than CRP. Recently, a broader view of SAA expression and function has been emerging. Expression studies show production of SAA proteins in histologically normal, atherosclerotic, Alzheimer, inflammatory, and tumor tissues. SAA has been found to have binding sites for high density lipoproteins, calcium, laminin, and heparin/heparan-sulfate. Also adhesion motifs were identified and new functions, affecting cell adhesion, migration, proliferation and aggregation discovered. These findings emphasize the importance of SAA in various physiological and pathological processes, including inflammation, atherosclerosis, thrombosis, AA-amyloidosis, rheumatoid arthritis, and neoplasia. SAA has also a number of immunomodulatory roles, it can induce chemotaxis and adhesion molecule expression, has cytokine-like properties and can promote the upregulation of metalloproteinases. It enhances the binding of high-density lipoprotein to macrophages and thus helps in the delivery of lipids to sites of injury for use in tissue repair. It is thus thought to be an integral part of the disease processes. In addition, recent experiments suggest that SAA may play a "housekeeping" role in normal human tissues. Elevated levels of SAA over time predispose to secondary amyloidosis, extracellular accumulation of amyloid fibrils, derived from a circulating precursor, in various tissue and organs. The most common form of amyloidosis occurs secondary to chronic inflammatory disease, particularly rheumatoid arthritis. The antibody is raised against human SAA and Helix Pomatia Haemocyanine. It reacts specifically with SAA-1, the major isoform of SAA in plasma.

Alternate Names

SAA, SAA1, SAA4, serum amyloid A1 cluster

Gene Symbol

SAA

Additional SAA Products

Product Documents for Human SAA ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human SAA ELISA Kit (Colorimetric)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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