Porcine Rotavirus Antigen ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-60133

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Feces (50 - 100 uL)

View all Rotavirus Antigen ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Porcine

Specificity

This assay has high sensitivity and excellent specificity for detection of pig Rotavirus antigen. No significant cross-reactivity or interference between pig Rotavirus antigen and analogues was observed.

Description

Assay Length: 1-5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 15

Inter-Assay Precision (Precision between assays) %CV < 15

Kit Contents for Porcine Rotavirus Antigen ELISA Kit (Colorimetric)

  • Adhesive Strip (For 96 wells)
  • Assay plate
  • HRP-conjugate
  • Instruction manual
  • Negative Control
  • Positive Control
  • Stop Solution
  • Substrate A
  • Substrate B
  • Wash Buffer

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Rotavirus Antigen

This assay employs the qualitative enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rotavirus antigen. Samples are pipetted into the wells with antibody conjugated Horseradish Peroxidase(HRP). Any Rotavirus antigen present in samples will bind to the pre-coated specific antibody. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of pig Rotavirus antigen bound in the initial step. The color development is stopped and the intensity of the color is measured.

Additional Rotavirus Antigen Products

Product Documents for Porcine Rotavirus Antigen ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Porcine Rotavirus Antigen ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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