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Prostaglandin E2 Parameter Assay Kit

Multi-species Prostaglandin E2/PGE2 ELISA Standard Curve
(2)
Multi-species Prostaglandin E2/PGE2 ELISA Linearity
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SKGE004B
PKGE004B
KGE004B

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Key Product Details

Assay Length: 3.5 hours
Sample Type & Volume Required Per Well: Cell Culture Supernates (51 uL), Serum (51 uL), EDTA Plasma (51 uL), Heparin Plasma (51 uL), Urine (51 uL)
Sample Types: Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine
Sensitivity: 41.4 pg/mL
Assay Range: 39 - 2,500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Prostaglandin E2 Parameter Assay Kit Summary: The Parameter PGE2 Immunoassay is a 3.5 hour forward sequential competitive enzyme immunoassay designed to measure PGE2 in cell culture supernates, serum, plasma, and urine.

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Customer Reviews (8)

3.9 out of 5
8 customer ratings
5 stars
38%
4 stars
38%
3 stars
0%
2 stars
25%
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  • Prostaglandin E2 Parameter Assay Kit
    Name: Anonymous
    Sample Tested: Serum
    Verified Customer | Posted 02/05/2022
    This kit is really great and easy to use!
    Prostaglandin E2 Parameter Assay Kit Serum KGE004B
  • Prostaglandin E2 Parameter Assay Kit
    Name: Anonymous
    Sample Tested: Cell Culture Samples
    Verified Customer | Posted 01/16/2022
    It performed really good and repeatable.
  • Prostaglandin E2 Parameter Assay Kit
    Name: Anonymous
    Sample Tested: Cell culture supernatant
    Verified Customer | Posted 04/03/2021
    High variability between replicates. Perhaps the most common issue with the kit is the requirement to complete the assay with a specific microplate shaker- which is how the assay was developed and QC tested, this is a horizontal orbital microplate shaker (0.12 inches or 3mm orbit) set at 500 ± 50 rpm.
    Prostaglandin E2 Parameter Assay Kit Serum KGE004B
    Bio-Techne Response
    Thank you for reviewing our product. We are sorry to that that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.
  • Prostaglandin E2 Parameter Assay Kit
    Name: Anonymous
    Sample Tested: Cell culture supernatant
    Verified Customer | Posted 11/04/2020
    I had trouble replicating the standard curve
    Prostaglandin E2 Parameter Assay Kit Serum KGE004B
    Bio-Techne Response
    Thank you for reviewing our product. We are sorry to that that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.
  • Prostaglandin E2 Parameter Assay Kit
    Name: Anonymous
    Sample Tested: Cell Culture Supernates
    Verified Customer | Posted 11/07/2019
    Prostaglandin E2 Parameter Assay Kit Serum KGE004B
  • Prostaglandin E2 Parameter Assay Kit
    Name: Anonymous
    Sample Tested: Cell Culture Media
    Verified Customer | Posted 11/03/2017
    Very easy to use, reliable results
  • Prostaglandin E2 Parameter Assay Kit
    Name: Anonymous
    Sample Tested: Adipose tissue
    Verified Customer | Posted 05/02/2017
  • Prostaglandin E2 Parameter Assay Kit
    Name: Anonymous
    Sample Tested: Mesenchymal stem cells
    Verified Customer | Posted 06/21/2016
    Prostaglandin E2 Parameter Assay Kit Serum KGE004B

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FAQs

  • How should the indomethacin be prepared, prior to being added to the serum or plasma collection tubes?

    First, dissolve indomethacin (Tocris cat # 1708) in ethanol to 10 mg/mL. Then, dilute indomethacin solution 1:50 in 1X PBS (to 0.2 mg/mL). Finally, inject 0.2 mg/mL indomethacin solution into vacutainer tube immediately following blood draw, to a final concentration of 10 μg/mL.

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.