Hi. Actually I want to purchase anti cPLA2 antibody raised in rabbit which can bind to both phorphorylated and unphosphorylated mouse cPLA2 group IV. I want to a gel shift assay with western blotting to show that my protein of interest is causing the phosphorylation of cPLA2 inside the mouse macrophages RAW cells.Can you please tell me which antibody would be suitable for the purpose. Thanks a lot.

Name: Human PLA2G4A ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP3-54972

Here are our antibodies raised in rabbit that have been validated to detect cPLA2 in mouse samples in a western blot. We have not specifically confirmed the ability of these antibodies to detect both the phosphorylated and unphosphorylated forms of the protein, and I cannot guarantee that they will clearly differentiate between the two.

Human PLA2G4A ELISA Kit (Colorimetric) New

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-54972

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Product Specifications
Precision
Scientific Data
Kit Contents
Preparation & Storage
Background
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Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, tissue homogenates and other biological fluids (100 uL)

Sensitivity

0.67 ng/mL (example only; lot dependent)

Assay Range

1.56 - 100 ng/mL (example only; lot dependent)

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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Description

Assay Length: 4.5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV < 12 (example only; lot dependent)

Scientific Data Images for Human PLA2G4A ELISA Kit (Colorimetric)

ELISA: Human PLA2G4A ELISA Kit (Colorimetric) [NBP3-54972]

Kit Contents for Human PLA2G4A ELISA Kit (Colorimetric)

Pre-coated 96T strip plate, Plate sealer for 96 wells, Standard, Diluent Buffer, Detection Reagent A, Detection Reagent B, TMB Substrate, Stop Solution, Wash Buffer (30 x concentrate), Instruction manual

Preparation and Storage

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: PLA2G4A

Secretory phopholipase A2 (PLA2) enzymes cleave an acyl ester bond in the sn-2 position of glycerophospholipids. These extracellular proteins have a high disulfide bond content, low molecular mass (14 kDa), and require mM levels of Ca2+ for catalysis. They play a crucial role in the generation of arachidonates and eicosanoids, and have a number of biological actions including immunological responses, inflammation, cellular proliferation, vasoconstriction, and bronchioconstriction.

Long Name

Phospholipase A2 Group IV A

Alternate Names

cPLA2, cPLA2-alpha

Gene Symbol

PLA2G4A

Additional PLA2G4A Products

Product Documents for Human PLA2G4A ELISA Kit (Colorimetric)

Product Datasheets

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Protocols & Manuals

Download Manual - NBP3-54972

COA

Product Specific Notices for Human PLA2G4A ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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