Phospho-HSP27 (S78/S82) DuoSet IC ELISA

Name: Phospho-HSP27 (S78/S82) DuoSet IC ELISA
Brand: R&D Systems
SKU: DYC2314-2

R&D Systems, Inc. a Bio-Techne Brand

Catalog # DYC2314-2

Citations (3)

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Key Product Details

Assay Type:	Solid Phase Sandwich ELISA
Assay Range:	62.5 - 4,000 pg/mL
Sample Types:	Cell Lysates
Phospho-HSP27 (S78/S82) DuoSet IC ELISA Features:	Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time Development protocols are provided to guide further assay optimization Assay can be customized to your specific needs Available in 2, 5, and 15- (96-well) plate pack sizes Economical alternative to Western blot

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Summary for Phospho-HSP27 (S78/S82) DuoSet IC ELISA

This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure phosphorylated human, mouse, and rat HSP27 (S78 / S82) in cell lysates. An immobilized capture antibody specific for HSP27 binds both phosphorylated and unphosphorylated HSP27. After washing away unbound material, a biotinylated detection antibody is used to detect only phosphorylated receptor, utilizing a standard HRP format.

Product Specifications for Phospho-HSP27 (S78/S82) DuoSet IC ELISA

Assay Format	96-well strip plate (sold separately)
Sample Volume Required	Cell lysates (100 µL)
Reactivity	Human, Mouse, Rat
Specificity	Please see the product datasheet
Detection Method	Colorimetric ELISA - 450nm (TMB)
Conjugate	Biotin
Label	HRP
Measurement	Quantitative ELISA
Sufficient Materials	Kits available for two, five, or fifteen 96-well plates*

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Kit Contents for Phospho-HSP27 (S78/S82) DuoSet IC ELISA

Capture Antibody
Conjugated Detection Antibody
Calibrated Immunoassay Standard or Control
Streptavidin-HRP

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2O4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Lysis Buffer*

IC Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: From Costar EIA Plate (Costar Catalog # 2592) or R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product

Scientific Data Examples for Phospho-HSP27 (S78/S82) DuoSet IC ELISA

	Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: HSP27

The heat shock proteins are a highly conserved family of stress response proteins. HSPs function primarily as molecular chaperones, facilitating the folding of other cellular proteins, preventing protein aggregation, or targeting improperly folded proteins to specific degradative pathways. Some HSPs are expressed at low levels under normal physiological conditions but show dramatically increased expression in response to cellular stress, others are constitutively expressed. Specific HSPs play a role in regulating apoptosis by interacting directly with key components of the apoptotic pathway.

Item	Value
Long Name	Heat Shock Protein 27
Entrez Gene IDs	3315 (Human); 15510 (Mouse); 24471 (Rat)
Alternate Names	28 kDa heat shock protein, DKFZp586P1322, Estrogen-regulated 24 kDa protein, HMN2B, HS.76067, HSP25, HSP27, HSP27HSP 27, HSP28CMT2F, HSPB1, Heat shock 27 kDa protein, SRP27, Stress-responsive protein 27, heat shock 27kD protein 1, heat shock 27kDa protein 1, heat shock protein beta-1

Citations for Phospho-HSP27 (S78/S82) DuoSet IC ELISA (3)

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Citations are publications that use Bio-Techne products. Selected citations for Phospho-HSP27 (S78/S82) DuoSet IC ELISA include:

Species: Human
Sample Types: Cell Lysates

OM David et al. (2022), The Stability and Anti-Angiogenic Properties of Titanium Dioxide Nanoparticles (TiO2NPs) Using Caco-2 Cells Biomolecules, 12(10).
PMID: 36291543
Species: Human
Sample Types: Serum

Schweiger T et al. (2015), Stromal expression of heat-shock protein 27 is associated with worse clinical outcome in patients with colorectal cancer lung metastases. PLoS ONE, 10(3):e0120724.
PMID: 25793600
Species: Human
Sample Types: Tissue Homogenates

Matt P et al. (2007), Proteomic alterations in heat shock protein 27 and identification of phosphoproteins in ascending aortic aneurysm associated with bicuspid and tricuspid aortic valve. J. Mol. Cell. Cardiol., 43(6):792-801.
PMID: 17949744

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