Mouse Tie-2 Quantikine ELISA Kit

Name: Mouse Tie-2 Quantikine ELISA Kit
Brand: R&D Systems
SKU: MTE200

R&D Systems, part of Bio-Techne | Catalog # MTE200

Submit a review

Product Datasheet / COA / SDS

Discontinued Product

MTE200 has been discontinued. View all Tie-2 products.

Key Product Details

Assay Length:	4.5 hours
Sample Type & Volume Required Per Well:	Cell Culture Supernates (50 uL), Tissue Homogenates (50 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Sensitivity:	40.2 pg/mL
Assay Range:	125 - 8,000 pg/mL (Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma, Heparin Plasma)
Mouse Tie-2 Quantikine ELISA Kit Summary:	The Quantikine Mouse Tie-2 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse Tie-2 in cell culture supernates, tissue homogenates, serum, and plasma. It contains NS0-expressed recombinant mouse Tie-2 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse Tie-2 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse Tie-2.

View all Tie-2 ELISA Kits »

View Full Technical Details for Mouse Tie-2 Quantikine ELISA Kit

Technical Data
Citations / Reviews

Product Specifications for Mouse Tie-2 Quantikine ELISA Kit

Assay Type	Solid Phase Sandwich ELISA
Format	96-well strip plate
Measurement	Quantitative ELISA
Detection Method	Colorimetric - 450nm (TMB)
Conjugate	HRP
Reactivity	Mouse
Specificity	Natural and recombinant mouse Tie-2
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	21	21	21
Mean (pg/mL)	395	1149	3470	434	1187	3183
Standard Deviation	26.1	69.1	220	34.5	77.6	147
CV%	6.6	6	6.3	7.9	6.5	4.6

Recovery

The recovery of mouse Tie-2 spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Supernates (n=4)	106	98-119
EDTA Plasma (n=5)	107	95-120
Heparin Plasma (n=4)	102	87-113
Serum (n=4)	103	93-116

Linearity

To assess the linearity of the assay, samples containing or spiked with recombinant mouse Tie-2 in each matrix were diluted with Calibrator Diluent and then assayed.

Scientific Data Examples for Mouse Tie-2 Quantikine ELISA Kit

	Mouse Tie-2 ELISA Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Tie-2

Tie-2, also known as Tek, is a transmembrane receptor tyrosine kinase (RTK) that functions as a receptor for Angiopoietin family proteins. It is expressed by embryonic and adult endothelial cells, hematopoietic stem cells, and a circulating population of proangiogenic monocytes. Tie-2 signaling is activated by Angiopoietins-1 and -4, while it can be either activated or inhibited by Angiopoietins-2 and -3. Tie-2 plays an important role in maintaining vascular integrity by mediating endothelial cell-smooth muscle cell communication and inhibiting endothelial cell apoptosis. It is also required for embryonic development of the endocardium. In addition, Tie-2 signaling mediates the quiescence of bone marrow stem cells in response to osteoblast-produced Angiopoietin-1. This quiescence is critical for maintaining an ongoing hematopoietic capability in the bone marrow.

Item	Value
Long Name	Tyrosine Kinase with Immunoglobulin and Epidermal Growth Factor Homology Domains 2
Entrez Gene IDs	7010 (Human); 21687 (Mouse); 89804 (Rat); 396729 (Porcine); 403714 (Canine); 102122204 (Cynomolgus Monkey); 30747 (Zebrafish)
Alternate Names	CD202b, CD202b antigen, EC 2.7.10, EC 2.7.10.1, TEK, TEK tyrosine kinase, endothelial, TIE2CD202b, Tie-2, Tie2, Tunica interna endothelial cell kinase, Tyrosine-protein kinase receptor TEK, Tyrosine-protein kinase receptor TIE-2, VMCM, VMCM1, angiopoietin-1 receptor, hTIE2, p140 TEK, soluble TIE2 variant 1, soluble TIE2 variant 2, venous malformations, multiple cutaneous and mucosal

Customer Reviews for Mouse Tie-2 Quantikine ELISA Kit

There are currently no reviews for this product. Be the first to review Mouse Tie-2 Quantikine ELISA Kit and earn rewards!

Have you used Mouse Tie-2 Quantikine ELISA Kit?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review

Product Documents for Mouse Tie-2 Quantikine ELISA Kit

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.