Mouse/Rat FABP1/L-FABP Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # RFBP10
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Urine (50 µL)
Sensitivity
0.029 ng/mL
Assay Range
0.078-5 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Product Summary for Mouse/Rat FABP1/L-FABP Quantikine ELISA Kit
The Quantikine Mouse/Rat FABP1 immunoassay is a 4.5 hour solid-phase ELISA designed to measure FABP1 in mouse or rat cell culture supernates, serum, plasma, and urine. It contains E. coli-expressed recombinant rat FABP1 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant rat FABP1. Results obtained using natural mouse or rat FABP1 showed dose response curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural FABP1.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Mouse, Rat
Specificity
Natural and recombinant FABP1
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 0.28 | 0.69 | 1.22 | 0.27 | 0.68 | 1.25 |
| Standard Deviation | 0.01 | 0.02 | 0.03 | 0.01 | 0.03 | 0.06 |
| CV% | 3.6 | 2.9 | 2.5 | 3.7 | 4.4 | 4.8 |
Recovery for Mouse/Rat FABP1/L-FABP Quantikine ELISA Kit
The recovery of FABP1 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 95 | 90-100 |
| Mouse Urine (n=4) | 96 | 86-108 |
| Rat EDTA Plasma (n=4) | 94 | 84-107 |
| Rat Heparin Plasma (n=4) | 100 | 92-111 |
| Rat Serum (n=4) | 100 | 87-116 |
| Rat Urine (n=4) | 99 | 95-105 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of FABP1 in each matrix were diluted with Calibrator Diluent and assayed.
Scientific Data Images for Mouse/Rat FABP1/L-FABP Quantikine ELISA Kit
Mouse/Rat FABP1/L-FABP ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: FABP1/L-FABP
Fatty Acid Binding Protein 1, also known as liver FABP, is a cytoplasmic transporter for long chain fatty acids, oxidized/peroxidized fatty acids, cholesterol, heme, Vitamin K, steroids, acyl CoA, leukotrienes, and prostaglandins. It is expressed by hepatocytes, simple columnar epithelium of the small intestine, and renal proximal tubule epithelium. FABP1 trafficks between the cytosol, peroxisomes, and nucleus. It delivers fatty acid cargo to the transcription factor PPAR alpha, leading to increased production of enzymes involved in the beta-oxidation of fatty acids. FABP1 also plays a role in the formation of prechylomicron vesicles by the endoplasmic reticulum.
Long Name
Fatty Acid-Binding Protein 1
Alternate Names
L-FABP, LFABP
Gene Symbol
FABP1
Additional FABP1/L-FABP Products
Product Documents for Mouse/Rat FABP1/L-FABP Quantikine ELISA Kit
Product Specific Notices for Mouse/Rat FABP1/L-FABP Quantikine ELISA Kit
For research use only
Loading...
Loading...
Loading...
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.