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Mouse/Rat FABP1/L-FABP Quantikine ELISA Kit

Bio-Techne includes R&D Systems | Catalog # RFBP10

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RFBP10

Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Urine (50 µL)

Sensitivity

0.029 ng/mL

Assay Range

0.078-5 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)

Product Summary for Mouse/Rat FABP1/L-FABP Quantikine ELISA Kit

The Quantikine Mouse/Rat FABP1 immunoassay is a 4.5 hour solid-phase ELISA designed to measure FABP1 in mouse or rat cell culture supernates, serum, plasma, and urine. It contains E. coli-expressed recombinant rat FABP1 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant rat FABP1. Results obtained using natural mouse or rat FABP1 showed dose response curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural FABP1.

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Reactivity

Mouse, Rat

Specificity

Natural and recombinant FABP1

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 0.28 0.69 1.22 0.27 0.68 1.25
Standard Deviation 0.01 0.02 0.03 0.01 0.03 0.06
CV% 3.6 2.9 2.5 3.7 4.4 4.8

Recovery for Mouse/Rat FABP1/L-FABP Quantikine ELISA Kit

The recovery of FABP1 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 95 90-100
Mouse Urine (n=4) 96 86-108
Rat EDTA Plasma (n=4) 94 84-107
Rat Heparin Plasma (n=4) 100 92-111
Rat Serum (n=4) 100 87-116
Rat Urine (n=4) 99 95-105

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of FABP1 in each matrix were diluted with Calibrator Diluent and assayed.

Mouse/Rat FABP1/L-FABP ELISA Linearity
Mouse/Rat FABP1/L-FABP ELISA Linearity

Scientific Data Images for Mouse/Rat FABP1/L-FABP Quantikine ELISA Kit

Mouse/Rat FABP1/L-FABP ELISA Standard Curve

Mouse/Rat FABP1/L-FABP ELISA Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: FABP1/L-FABP

Fatty Acid Binding Protein 1, also known as liver FABP, is a cytoplasmic transporter for long chain fatty acids, oxidized/peroxidized fatty acids, cholesterol, heme, Vitamin K, steroids, acyl CoA, leukotrienes, and prostaglandins. It is expressed by hepatocytes, simple columnar epithelium of the small intestine, and renal proximal tubule epithelium. FABP1 trafficks between the cytosol, peroxisomes, and nucleus. It delivers fatty acid cargo to the transcription factor PPAR alpha, leading to increased production of enzymes involved in the beta-oxidation of fatty acids. FABP1 also plays a role in the formation of prechylomicron vesicles by the endoplasmic reticulum.

Long Name

Fatty Acid-Binding Protein 1

Alternate Names

L-FABP, LFABP

Entrez Gene IDs

2168 (Human); 14080 (Mouse); 24360 (Rat)

Gene Symbol

FABP1

Additional FABP1/L-FABP Products

Product Documents for Mouse/Rat FABP1/L-FABP Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse/Rat FABP1/L-FABP Quantikine ELISA Kit

For research use only

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Protocols

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.