Mouse IFN-alpha All Subtype Quantikine ELISA Kit

Name: Mouse IFN-alpha All Subtype Quantikine ELISA Kit
Brand: R&D Systems
SKU: MFNAS0
Price: 635 EUR

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MFNAS0

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Assay Length:	3.5 hours
Sample Type & Volume Required Per Well:	Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
Sample Types:	Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
Sensitivity:	3.79 pg/mL
Assay Range:	15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Mouse IFN-alpha All Subtype Quantikine ELISA Kit Summary:	The Quantikine™ Mouse IFN-alpha All Subtype immunoassay is a 3.5 hour solid-phase ELISA designed to measure IFN-alpha in mouse cell culture supernates, serum, and plasma. It contains HEK293-expressed recombinant mouse IFN-alpha and has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse IFN-alpha showed dose response curves that were parallel to the standard curves obtained using the Quantikine mouse kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IFN-alpha.

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Product Specifications for Mouse IFN-alpha All Subtype Quantikine ELISA Kit

Measurement	Quantitative ELISA
Detection Method	Colorimetric - 450nm (TMB)
Conjugate	HRP
Reactivity	Mouse
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using one lot of kit components

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	82.2	590	199	79.3	602	195
Standard Deviation	3.04	15.9	3.08	5.11	57.4	7.93
CV%	3.7	2.7	1.5	6.4	9.5	4.1

Recovery

The recovery of mouse IFN-alpha spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	98	91-106
EDTA Plasma (n=4)	105	91-121
Heparin Plasma (n=4)	106	96-120
Serum (n=4)	102	86-118

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse IFN-alpha were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.

Scientific Data Examples for Mouse IFN-alpha All Subtype Quantikine ELISA Kit

	Mouse IFN-alpha All Subtype Quantikine Natural Linearity Competitor Comparison IFN-alpha samples containing a high concentration in various matrices are diluted with appropriate Calibrator Diluent to produce sample values within the dynamic range of the assay. R&D Systems natural linearity is between 98%-103% compared to 125%-180% for the top competitor.
	Mouse IFN-alpha All Subtype Quantikine ELISA Spiked Linearity Competitor Comparison IFN-alpha is spiked at high concentration in various matrices and diluted with appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay. R&D Systems linearity is between 88%-92% compared to 132%-201% for the top competitor.
	Mouse IFN-alpha All Subtype Quantikine ELISA Spiked Recovery IFN-alpha is spiked at three known concentrations throughout the range of the assay and run to measure response of the spiked sample matrix. R&D Systems recovery is 99%.
	Mouse IFN-alpha All Subtype ELISA Standard Curve

Preparation and Storage

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IFN-alpha

Interferon-alpha (IFN-alpha), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits, a 100 kDa ligand-binding subunit (IFN-alpha R2) and a 125 kDa ligand binding and signal transduction subunit (IFN-alpha R1) that is involved both in ligand binding and signal transduction. IFN-alpha has both anti-viral and immunomodulatory activities on target cells.

Item	Value
Long Name	Interferon alpha
Entrez Gene IDs	3440 (Human)
Alternate Names	IFN-alpha, IFN-alphaA, IFNA, IFNA2, IFNA2b, IFNalpha, INFA2, LeIF D, interferon alpha 2

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Mouse IFN-alpha All Subtype Quantikine ELISA Kit

R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Mouse IFN-alpha All Subtype Quantikine ELISA Kit

Precision

Recovery

Linearity

Scientific Data Examples for Mouse IFN-alpha All Subtype Quantikine ELISA Kit

Preparation and Storage

Background: IFN-alpha

Citations for Mouse IFN-alpha All Subtype Quantikine ELISA Kit

Product Documents for Mouse IFN-alpha All Subtype Quantikine ELISA Kit

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