Human, Mouse, Rat Methotrexate ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-62161

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NBP2-62161
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Key Product Details

Sample Type & Volume Required Per Well

Human, mouse, and rat serum, plasma, urine (100 uL)

Sensitivity

0.087 ng/ml

Assay Range

0.13-1000 ng/ml

View all Methotrexate ELISA Kits »

Product Specifications

Assay Type

Competitive ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human, Mouse, Rat

Precision

Intra-Assay Precision (Precision within an assay) Low Concentration %CV - 20.5% High Concentration %CV - 9.8%

Inter-Assay Precision (Precision between assays) Low Concentration %CV - 22.4% High Concentration %CV - 17.1%

Recovery for Human, Mouse, Rat Methotrexate ELISA Kit (Colorimetric)

Recovery

Human Serum Low - 71%
Medium - 89%
High - 86%

Human Plasma Low - 91%
Medium - 107%
High - 77%

Mouse Serum Low - 141%
Medium - 140%
High - 135%

Mouse Plasma Low - 90%
Medium - 168%
High - 56%

Scientific Data Images for Human, Mouse, Rat Methotrexate ELISA Kit (Colorimetric)

ELISA: Human, Mouse, Rat Methotrexate ELISA Kit (Colorimetric) [NBP2-62161]

ELISA: Human, Mouse, Rat Methotrexate ELISA Kit (Colorimetric) [NBP2-62161]

ELISA: Human, Mouse, Rat Methotrexate ELISA Kit (Colorimetric) [NBP2-62161] - A typical standard curve for Methotrexate ELISA kit. These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Kit Contents for Human, Mouse, Rat Methotrexate ELISA Kit (Colorimetric)

  • and Stop Solution
  • Antibody
  • Antibody Diluent
  • Assay Buffer 13
  • Conjugate
  • Microtiter Plate
  • Standard
  • Substrate
  • Wash Buffer Concentrate

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4°C.

Background: Methotrexate

Alternate Names

Amethopterine, MTX

Additional Methotrexate Products

Product Documents for Human, Mouse, Rat Methotrexate ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human, Mouse, Rat Methotrexate ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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