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Human LL-37 ELISA Kit (Colorimetric)

Catalog # NBP3-06932 | Novus Biologicals a Bio-Techne Brand
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NBP3-06932

Key Product Details

Sample Type & Volume Required Per Well

Serum, Plasma, Cell supernatant (100 ul)

Sensitivity

0.94 ng/mL

Assay Range

1.56 - 100 ng/mL

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This kit recognizes Human LL-37 in samples. No significant cross-reactivity or interference between Human LL-37 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) 5.51

Inter-Assay Precision (Precision between assays) 4.46

Recovery for Human LL-37 ELISA Kit (Colorimetric)

Recovery

92-109 %

Linearity

Human LL-37 ELISA Kit (Colorimetric)

Scientific Data Images for Human LL-37 ELISA Kit (Colorimetric)

ELISA: Human LL-37 ELISA Kit (Colorimetric) [NBP3-06932] - Standard Curve Reference.

Kit Contents for Human LL-37 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Certificate of Analysis
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate(Dismountable)
  • Plate Sealer
  • Product Description
  • Reference Standard
  • Reference Standard & Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: LL-37

Cathelicidins are a family of antimicrobial proteins predominantly found in the peroxidase negative granules of neutrophils. The cathelicidins are synthesized as preproproteins. Within the neutrophils, they are stored in granules as inactive proforms after removal of the signal peptide. The active biologic domains of the cathelicidins generally reside in the C terminus. The C terminal antimicrobial peptides are activated when cleaved from the proforms of the cathelicidins by serine proteases from azurophil granules. FUNCTION: Binds to bacterial lipopolysaccharides (LPS), has antibacterial activity. SUBCELLULAR LOCATION: Secreted. TISSUE SPECIFICITY: Expressed in bone marrow and testis and neutrophils.

Alternate Names

18 kDa cationic antimicrobial protein, Antibacterial peptide FALL-39, CAP18, CAP-18, cathelicidin antimicrobial peptide, CRAMP, epididymis secretory sperm binding protein, FALL39, FALL-39, FALL-39 peptide antibiotic, hCAP-181, HSD26, LL37

Gene Symbol

CAMP

Product Documents for Human LL-37 ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human LL-37 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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