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Rat IL-1ra/IL-1F3/IL1RN ELISA Kit (Colorimetric), Novus Biologicals

Bio-Techne includes Novus Biologicals | Catalog # NBP3-06804

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NBP3-06804

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell supernatant, and other biological fluids (100 ul)

Sensitivity

46.88 pg/mL

Assay Range

78.13 - 5000 pg/mL

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This kit recognizes Rat IL-1ra/IL-1F3/IL1RN in samples. No significant cross-reactivity or interference between Rat IL-1ra/IL-1F3/IL1RN and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) 4.51

Inter-Assay Precision (Precision between assays) 5.66

Recovery for Rat IL-1ra/IL-1F3/IL1RN ELISA Kit (Colorimetric)

Recovery

86 - 105 %

Linearity

Rat IL-1ra/IL-1F3/IL1RN ELISA Kit (Colorimetric)

Scientific Data Images for Rat IL-1ra/IL-1F3/IL1RN ELISA Kit (Colorimetric)

Rat IL-1ra/IL-1F3/IL1RN ELISA Kit (Colorimetric)

ELISA: Rat IL-1ra/IL-1F3/IL1RN ELISA Kit (Colorimetric) [NBP3-06804] -

ELISA: Rat IL-1ra/IL-1F3/IL1RN ELISA Kit (Colorimetric) [NBP3-06804] - Standard Curve Reference

Kit Contents for Rat IL-1ra/IL-1F3/IL1RN ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Certificate of Analysis
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate(Dismountable)
  • Plate Sealer
  • Product Description
  • Reference Standard
  • Reference Standard & Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: IL-1ra/IL-1F3

IL-1ra (IL-1 receptor antagonist) is a widely expressed acute phase protein that serves to dampen inflammation. IL-1ra binds to both IL-1 RI and IL-1 RII. It competitively inhibits IL-1 (alpha or beta) binding to IL-1 RI and does not trigger recruitment of the accessory molecule IL-1 R AcP or activation of IL-1 RI signaling. IL-1ra blocks the proinflammatory actions of IL-1 including Prostaglandin E2 and IL-6 release from endothelial cells, fever generation, thrombocytosis, the production of hepatic acute phase proteins, and neutrophil expansion and infiltration.

Long Name

Interleukin 1 Receptor Antagonist

Alternate Names

DIRA, ICIL-1ra, IL-1F3, IL-1ra3, IL-1RN, IL1ra, IL1RN, MVCD4

Gene Symbol

IL1RN

Additional IL-1ra/IL-1F3 Products

Product Documents for Rat IL-1ra/IL-1F3/IL1RN ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat IL-1ra/IL-1F3/IL1RN ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

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