Human Total PON1 DuoSet IC ELISA

Name: Human Total PON1 DuoSet IC ELISA
Brand: R&D Systems
SKU: DYC5816-2

R&D Systems, Inc. a Bio-Techne Brand

Catalog # DYC5816-2

Citations (4)

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Key Product Details

Assay Type:	Solid Phase Sandwich ELISA
Assay Range:	156 - 10,000 pg/mL
Sample Types:	Cell Lysates
Human Total PON1 DuoSet IC ELISA Features:	Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time Development protocols are provided to guide further assay optimization Assay can be customized to your specific needs Available in 2, 5, and 15- (96-well) plate pack sizes Economical alternative to Western blot

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Summary for Human Total PON1 DuoSet IC ELISA

This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure total PON1 in cell lysates. An immobilized capture antibody specific for PON1 binds both phosphorylated and unphosphorylated PON1. After washing away unbound material, a biotinylated detection antibody is used to detect both phosphorylated and unphosphorylated protein, utilizing a standard Streptavidin-HRP format.

Product Specifications for Human Total PON1 DuoSet IC ELISA

Assay Format	96-well strip plate (sold separately)
Sample Volume Required	Cell lysates (100 µL)
Reactivity	Human
Specificity	Please see the product datasheet
Detection Method	Colorimetric ELISA - 450nm (TMB)
Conjugate	Biotin
Label	HRP
Measurement	Quantitative ELISA
Sufficient Materials	Kits available for two, five, or fifteen 96-well plates*

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Kit Contents for Human Total PON1 DuoSet IC ELISA

Capture Antibody
Conjugated Detection Antibody
Calibrated Immunoassay Standard or Control
Streptavidin-HRP

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2O4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Lysis Buffer*

IC Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: From Costar EIA Plate (Costar Catalog # 2592) or R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product

Scientific Data Examples for Human Total PON1 DuoSet IC ELISA

	Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: PON1

PON (paraoxonase/lactonase) family members PON1, PON2 and PON3 are calcium-dependent antioxidant enzymes. PON1 is secreted into the bloodstream associated with high-density lipoproteins (HDL). Serum PON1 concentrations vary widely among normal individuals, in part due to differential expression of some polymorphisms. The antioxidant activity of PON1 and other PON members may slow the initiation and progression of atherosclerosis.

Item	Value
Long Name	Paraoxonase 1
Entrez Gene IDs	5444 (Human); 18979 (Mouse); 84024 (Rat)
Alternate Names	A-esterase 1, Aromatic esterase 1, EC 3.1.1.2, EC 3.1.1.81, EC 3.1.8.1, ESA, ESAesterase A, K-45, MVCD5, PON 1, PON1, PONparaoxonase B-type, Serum aryldialkylphosphatase 1, arylesterase B-type, paraoxonase 1, serum aryldiakylphosphatase, serum paraoxonase/arylesterase 1

Citations for Human Total PON1 DuoSet IC ELISA (4)

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Citations are publications that use Bio-Techne products. Selected citations for Human Total PON1 DuoSet IC ELISA include:

Species: Human
Sample Types: Plasma

T Barbalata et al. (2020), Increased miR-142 Levels in Plasma and Atherosclerotic Plaques from Peripheral Artery Disease Patients with Post-Surgery Cardiovascular Events International Journal of Molecular Sciences, 21(24).
PMID: 33339419
Species: Human
Sample Types: Plasma

CJ Mohammed et al. (2019), Circulating Lactonase Activity but Not Protein Level of PON-1 Predicts Adverse Outcomes in Subjects with Chronic Kidney Disease J Clin Med, 8(7).
PMID: 31311140
Species: Human
Sample Types: Plasma

NK Sharma et al. (2019), Lipid metabolism impairment in patients with sepsis secondary to hospital acquired pneumonia, a proteomic analysis Clin Proteomics, 16(0):29.
PMID: 31341447
Species: Human
Sample Types: Serum

MG Carnuta et al. (2017), Dysfunctional high-density lipoproteins have distinct composition, diminished anti-inflammatory potential and discriminate acute coronary syndrome from stable coronary artery disease patients Sci Rep, 7(1):7295.
PMID: 28779156

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