Human Total p21/CIP1/CDKN1A DuoSet IC ELISA

Name: Human Total p21/CIP1/CDKN1A DuoSet IC ELISA
Brand: R&D Systems
SKU: DYC1047-2

R&D Systems, Inc. a Bio-Techne Brand

Catalog # DYC1047-2

Citations (3)

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Key Product Details

Assay Type:	Solid Phase Sandwich ELISA
Assay Range:	62.5 - 4,000 pg/mL
Sample Types:	Cell Lysates
Human Total p21/CIP1/CDKN1A DuoSet IC ELISA Features:	Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time Development protocols are provided to guide further assay optimization Assay can be customized to your specific needs Available in 2, 5, and 15- (96-well) plate pack sizes Economical alternative to Western blot

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Summary for Human Total p21/CIP1/CDKN1A DuoSet IC ELISA

This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure total p21 / CIP1 / CDKN1A in cell lysates. An immobilized capture antibody specific for p21 / CIP1 / CDKN1A binds both phosphorylated and unphosphorylated p21 / CIP1 / CDKN1A. After washing away unbound material, a biotinylated detection antibody is used to detect both phosphorylated and unphosphorylated protein, utilizing a standard Streptavidin-HRP format.

Product Specifications for Human Total p21/CIP1/CDKN1A DuoSet IC ELISA

Assay Format	96-well strip plate (sold separately)
Sample Volume Required	Cell lysates (100 µL)
Reactivity	Human
Specificity	Please see the product datasheet
Detection Method	Colorimetric ELISA - 450nm (TMB)
Conjugate	Biotin
Label	HRP
Measurement	Quantitative ELISA
Sufficient Materials	Kits available for two, five, or fifteen 96-well plates*

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Kit Contents for Human Total p21/CIP1/CDKN1A DuoSet IC ELISA

Capture Antibody
Conjugated Detection Antibody
Calibrated Immunoassay Standard or Control
Streptavidin-HRP

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2O4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Lysis Buffer*

IC Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: From Costar EIA Plate (Costar Catalog # 2592) or R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product

Scientific Data Examples for Human Total p21/CIP1/CDKN1A DuoSet IC ELISA

	Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: p21/CIP1/CDKN1A

p21/CIP1 is a Cyclin/Cyclin-dependent kinase (Cdk) inhibitor that blocks progression from G1 to S phase in the cell cycle. The expression of p21, a transcriptional target of p53, increases in cells where p53 is stabilized due to exposure to genotoxic agents.

Item	Value
Long Name	p21 Cyclin Dependent Kinase 4 Inhibitor 1A
Entrez Gene IDs	1026 (Human); 12575 (Mouse); 114851 (Rat)
Alternate Names	CAP20cyclin-dependent kinase inhibitor 1, CDK-interacting protein 1, CDKN1A, CDKN1melanoma differentiation associated protein 6, CIP1, CIP1WAF1CDK-interaction protein 1, MDA-6, MDA6, Melanoma differentiation-associated protein 6, PIC1, SDI1DNA synthesis inhibitor, cyclin-dependent kinase inhibitor 1A (p21, Cip1), p21, p21CIP1, p21Cip1/Waf1, wild-type p53-activated fragment 1

Citations for Human Total p21/CIP1/CDKN1A DuoSet IC ELISA (3)

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Citations are publications that use Bio-Techne products. Selected citations for Human Total p21/CIP1/CDKN1A DuoSet IC ELISA include:

Species: Human
Sample Types: Cell Lysates

M Bostan et al. (2020), The Effect of Resveratrol or Curcumin on Head and Neck Cancer Cells Sensitivity to the Cytotoxic Effects of Cisplatin Nutrients, 12(9).
PMID: 32859062
Species: Human
Sample Types: Cell Lysates

M Bostan et al. (2020), The Effect of Resveratrol or Curcumin on Head and Neck Cancer Cells Sensitivity to the Cytotoxic Effects of Cisplatin Nutrients, 12(9).
PMID: 32859062
Species: Human
Sample Types: Cell Lysates

Eckstein N et al. (2007), Epidermal growth factor receptor pathway analysis identifies amphiregulin as a key factor for cisplatin resistance of human breast cancer cells. J. Biol. Chem., 283(2):739-50.
PMID: 17942395

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